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GANAB Recombinant Rabbit Monoclonal Antibody [PSH01-53]

Usd: 385

Specification

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Catalog# HA721696

GANAB Recombinant Rabbit Monoclonal Antibody [PSH01-53]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

GANAB Recombinant Rabbit Monoclonal Antibody [PSH01-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GANAB aa 29-450.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 107 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, LoVo cell lysate, HEK-293 cell lysate, U-2 OS cell lysate, K-562 cell lysate, Jurkat cell lysate, HL-60 cell lysate, Raji cell lysate, human thyroid tissue, human liver tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

PSH01-53

RRID

AB_3072809

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. atalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.

Background References

1. Pelletier M.F., Marcil A., Sevigny G., Jakob C.A., Tessier D.C., Chevet E., Menard R., Bergeron J.J.M., Thomas D.Y. The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. Glycobiology 10:815-827 (2000)

2. Porath B., Gainullin V.G., Cornec-Le Gall E., Dillinger E.K., Heyer C.M., Hopp K., Edwards M.E., Madsen C.D., et al. Mutations in GANAB, encoding the glucosidase IIalpha subunit, cause autosomal-dominant polycystic kidney and liver disease. Am. J. Hum. Genet. 98:1193-1207 (2016)

Subcellular Location

Endoplasmic reticulum, Golgi apparatus.

UNIPROT

SwissProt: Q14697 Human

Synonyms

Alpha glucosidase II alpha subunit antibody

Alpha-glucosidase 2 antibody

G2AN antibody

GANAB antibody

GANAB_HUMAN antibody

Glu II antibody

Glucosidase alpha neutral AB antibody

Glucosidase II alpha antibody

Glucosidase II subunit alpha antibody

GluII antibody

Expand

Images

  • Western blot analysis of GANAB on different lysates with Rabbit anti-GANAB antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: LoVo cell lysate (20 µg/Lane)<br />Lane 4: HEK-293 cell lysate (20 µg/Lane)<br />Lane 5: U-2 OS cell lysate (20 µg/Lane)<br />Lane 6: K-562 cell lysate (20 µg/Lane)<br />Lane 7: Jurkat cell lysate (20 µg/Lane)<br />Lane 8: HL-60 cell lysate (20 µg/Lane)<br />Lane 9: Raji cell lysate (20 µg/Lane)<br /><br />Predicted band size: 107 kDa<br />Observed band size: 107 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GANAB on different lysates with Rabbit anti-GANAB antibody (HA721696) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: LoVo cell lysate (20 µg/Lane)
    Lane 4: HEK-293 cell lysate (20 µg/Lane)
    Lane 5: U-2 OS cell lysate (20 µg/Lane)
    Lane 6: K-562 cell lysate (20 µg/Lane)
    Lane 7: Jurkat cell lysate (20 µg/Lane)
    Lane 8: HL-60 cell lysate (20 µg/Lane)
    Lane 9: Raji cell lysate (20 µg/Lane)

    Predicted band size: 107 kDa
    Observed band size: 107 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721696) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling GANAB with Rabbit anti-GANAB antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling GANAB with Rabbit anti-GANAB antibody (HA721696) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721696, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GANAB antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GANAB antibody (HA721696) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721696) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GANAB antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GANAB antibody (HA721696) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721696) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-GANAB antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721696" style="font-weight: bold;text-decoration: underline;">HA721696</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-GANAB antibody (HA721696) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721696) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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