RACGAP1 Recombinant Rabbit Monoclonal Antibody [JE41-19]
Catalog# HA721687
RACGAP1 Recombinant Rabbit Monoclonal Antibody [JE41-19]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
RACGAP1 Recombinant Rabbit Monoclonal Antibody [JE41-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human RACGAP1 aa 51-100 / 632.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 71 kDa
Positive Control
Jurkat cell lysate, K-562 cell lysate, 293T cell lysate, HeLa cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate.
Conjugation
unconjugated
Clone Number
JE41-19
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Rac GTPase-activating protein 1 is an enzyme that in humans is encoded by the RACGAP1 gene. Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases. Over-expression of RACGAP1 is observed in multiple human cancers including breast cancer, gastric cancer and colorectal cancer. Evidence show that RACGAP1 can modulate mitochondrial quality control by stimulating mitopahy and mitochondrial biogenesis in breast cancer. Knocking out RACGAP1 in vitro using CRISPR/Cas9 leads to cytokinesis failure.
Background References
1. Eid RA et al. Assessment of RACGAP1 as a Prognostic and Immunological Biomarker in Multiple Human Tumors: A Multiomics Analysis. Int J Mol Sci. 2022 Nov
2. Ren K et al. RACGAP1 modulates ECT2-Dependent mitochondrial quality control to drive breast cancer metastasis. Exp Cell Res. 2021 Mar
Subcellular Location
Nucleus, Cytoplasm, cytoskeleton, spindle, Cytoplasmic vesicle, secretory vesicle, acrosome, Cleavage furrow, Midbody, Midbody ring, Cell membrane.
Synonyms
CYK4 antibody
GAP antibody
Gap1 antibody
GTPase activating protein antibody
HsCYK-4 antibody
ID GAP antibody
KIAA1478 antibody
Male germ cell RacGap antibody
MgcRacGAP antibody
Protein CYK4 homolg antibody
ExpandImages
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Western blot analysis of RACGAP1 on different lysates with Rabbit anti-RACGAP1 antibody (HA721687) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: PC-12 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721687) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"