Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE58-32]
Catalog# HA721676
Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE58-32]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE58-32]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 69 kDa
Positive Control
NIH/3T3 cell lysate, HeLa cell lysate.
Conjugation
unconjugated
Clone Number
JE58-32
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Eukaryotic translation initiation factor 4B is a protein that in humans is encoded by the EIF4B gene. eIF4B has been shown to interact with and stimulate the activity of eIF4A and bind to the eIF3 complex through the eIF3A subunit. This interaction results in the recruitment of the eukaryotic small ribosomal subunit (40S) to the mRNA which will in turn set the stage for the later steps leading to elongation.
Background References
1. Pontheaux F et al. eIF4B mRNA Translation Contributes to Cleavage Dynamics in Early Sea Urchin Embryos. Biology (Basel). 2022 Sep
2. Kapadia BB et al. PARK2 regulates eIF4B-driven lymphomagenesis. Mol Cancer Res. 2022 Feb
Subcellular Location
cytosol, eukaryotic translation initiation factor 4F complex.
Synonyms
2310046H11Rik antibody
AL024095 antibody
C85189 antibody
EIF 4B antibody
eIF-4B antibody
EIF4B antibody
Eukaryotic initiation factor 4B antibody
Eukaryotic translation initiation factor 4B antibody
IF4B_HUMAN antibody
PRO1843 antibody
Images
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☑ Cell treatment (CT)
Western blot analysis of Phospho-eIF4B (S406) on different lysates with Rabbit anti-Phospho-eIF4B (S406) antibody (HA721676) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 treated λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 80 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721676) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"