Thyroid Hormone Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE40-49]
Catalog# HA721663
Thyroid Hormone Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE40-49]
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WB
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Human
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unconjugated
Overview
Product Name
Thyroid Hormone Receptor alpha Recombinant Rabbit Monoclonal Antibody [JE40-49]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Thyroid Hormone Receptor alpha aa 416-465 / 490.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 55 kDa
Positive Control
A431 cell lysate, SW480 cell lysate.
Conjugation
unconjugated
Clone Number
JE40-49
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Thyroid hormone receptor alpha (TR-alpha) also known as nuclear receptor subfamily 1, group A, member 1 (NR1A1), is a nuclear receptor protein that in humans is encoded by the THRA gene. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Mutations of the THRA gene may cause nongoitrous congenital hypothyroidism-6, a subtype of congenital hypothyroidism.
Background References
1. Liu S et al. Triiodothyronine (T3) promotes brown fat hyperplasia via thyroid hormone receptor α mediated adipocyte progenitor cell proliferation. Nat Commun. 2022 Jun
2. Han CR et al. Thyroid Hormone Receptor α Mutations Cause Heart Defects in Zebrafish. Thyroid. 2021 Feb
Subcellular Location
Nucleus; Cytoplasm, Nucleus.
UNIPROT
Synonyms
AR7 antibody
c-erbA-1 antibody
C-erbA-alpha antibody
CHNG6 antibody
EAR-7 antibody
EAR7 antibody
ERB-T-1 antibody
ERBA antibody
ERBA-related 7 antibody
ERBA1 antibody
ExpandImages
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Western blot analysis of Thyroid Hormone Receptor alpha on different lysates with Rabbit anti-Thyroid Hormone Receptor alpha antibody (HA721663) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721663) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"