GAS2 Recombinant Rabbit Monoclonal Antibody [JE48-38]
Catalog# HA721647
GAS2 Recombinant Rabbit Monoclonal Antibody [JE48-38]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
GAS2 Recombinant Rabbit Monoclonal Antibody [JE48-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human GAS2 aa 1-50 / 313.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
Jurkat cell lysate, NIH/3T3 cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, human stomach tissue, mouse liver tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
JE48-38
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:200-1:1,000
Target
Function
Growth arrest-specific protein 2 is a protein that in humans is encoded by the GAS2 gene. The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
Background References
1. Chen T et al. Cochlear supporting cells require GAS2 for cytoskeletal architecture and hearing. Dev Cell. 2021 May
2. Kong Y et al. GAS2 Promotes Cell Proliferation and Invasion and Suppresses Apoptosis in Pediatric T-Cell Acute Lymphoblastic Leukemia and Activates Wnt/β-Catenin Pathway. Onco Targets Ther. 2020 Feb
Subcellular Location
Cytoplasm, cytoskeleton, stress fiber, Membrane.
Synonyms
GAS 2 antibody
GAS-2 antibody
Gas2 antibody
GAS2_HUMAN antibody
Growth arrest specific 2 antibody
Growth arrest specific protein 2 antibody
Growth arrest-specific protein 2 antibody
MGC32610 antibody
Images
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Western blot analysis of GAS2 on different lysates with Rabbit anti-GAS2 antibody (HA721647) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human liver tissue lysate (40 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721647) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-GAS2 antibody (HA721647) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721647) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GAS2 antibody (HA721647) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721647) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GAS2 antibody (HA721647) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721647) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"