Legumain Recombinant Rabbit Monoclonal Antibody [JE35-56]
Catalog# HA721645
Legumain Recombinant Rabbit Monoclonal Antibody [JE35-56]
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WB
-
IHC-P
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
Legumain Recombinant Rabbit Monoclonal Antibody [JE35-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Legumain aa 151-350 / 433.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 49 kDa
Positive Control
HT-29 cell lysate, HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, Neuro-2a cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, mouse kidney tissue lysate, mouse placenta tissue lysate, rat kidney tissue lysate, human kidney tissue lysate, human kidney tissue, human placenta tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
JE35-56
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:200-1:1,000
Target
Function
Legumain is a protein that in humans is encoded by the LGMN gene. This gene encodes a cysteine protease, legumain, that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
Background References
1. Chen C et al. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI. Cell Death Dis. 2021 Jan
2. Jia D et al. Cardiac Resident Macrophage-Derived Legumain Improves Cardiac Repair by Promoting Clearance and Degradation of Apoptotic Cardiomyocytes After Myocardial Infarction. Circulation. 2022 May
Subcellular Location
Lysosome.
Synonyms
AEP antibody
Asparaginyl endopeptidase antibody
cysteine 1 antibody
Cysteine protease 1 antibody
EC 3.4.22.34 antibody
Legumain antibody
LGMN antibody
LGMN_HUMAN antibody
LGMN1 antibody
Protease antibody
ExpandImages
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Western blot analysis of Legumain on different lysates with Rabbit anti-Legumain antibody (HA721645) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: RAW264.7 cell lysate (20 µg/Lane)
Lane 8: Mouse kidney tissue lysate (40 µg/Lane)
Lane 9: Mouse placenta tissue lysate (40 µg/Lane)
Lane 10: Rat kidney tissue lysate (40 µg/Lane)
Lane 11: Human kidney tissue lysate (40 µg/Lane)
Predicted band size: 49 kDa
Observed band size: 36/46/56 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721645) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Legumain antibody (HA721645) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721645) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Legumain antibody (HA721645) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721645) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Legumain antibody (HA721645) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721645) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"