MSI2 Recombinant Rabbit Monoclonal Antibody [PSH01-17]
Catalog# HA721634
MSI2 Recombinant Rabbit Monoclonal Antibody [PSH01-17]
-
WB
-
IHC-P
-
IF-Cell
-
IP
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
MSI2 Recombinant Rabbit Monoclonal Antibody [PSH01-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MSI2 aa 1-185 / 328.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, IP
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
PC-12 cell lysate, K-562 cell lysate, human placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat testis tissue lysate, mouse brain tissue, rat brain tissue, HeLa.
Conjugation
unconjugated
Clone Number
PSH01-17
RRID
Reactivity Data
| WB | IHC-P | IF-Cell | IP | |
|---|---|---|---|---|
| Human |
|
|
||
| Mouse |
|
|
||
| Rat |
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
-
IF-Cell
-
1:100
-
IP
-
1-2μg/sample
Target
Function
This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is translational regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells. The first musashi (abbreviation MSI) gene was first discovered in Drosophila and then later identified in other eukaryotic species. MSI2 is involved in organismal development. As with the rest of Musashi family RNA-binding proteins, MSI2 is linked to tissue stem cells and has an influence in asymmetric cell division, germ and somatic stem cell function and cell fate determination in a variety of tissues. As an RNA-binding protein, MSI2 is acts as a translational inhibitor. Through this molecular mechanism, MSI2 contributes in more than one vital aspect, as in the development of the nervous system, regulation of the Hematopoietic stem cell (HSC) compartment, or the self-renewal and pluripotency of embryonic stem cells. MSI2 takes part in a high number of pathways related to the self-renewal of some stem cells. However, it is not only focused in one specific type. Depending on the tissue where it is located, it develops different functions.
Background References
1. Zhu Y et al. LncRNA LINC00942 promotes chemoresistance in gastric cancer by suppressing MSI2 degradation to enhance c-Myc mRNA stability. Clin Transl Med. 2022 Jan
2. Qu C et al. Myofibroblast-Specific Msi2 Knockout Inhibits HCC Progression in a Mouse Model. Hepatology. 2021 Jul
Subcellular Location
Cytoplasm.
Synonyms
FLJ36569 antibody
MGC3245 antibody
Msi2 antibody
MSI2/HOXA9 fusion gene, included antibody
MSI2H antibody
MSI2H_HUMAN antibody
Musashi 2 antibody
Musashi homolog 2 antibody
Musashi RNA binding protein 2 antibody
Musashi, Drosophila, homolog of, 2 antibody
ExpandImages
-
Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (HA721634) at 1/1,000 dilution.
Lane 1: PC-12 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Human placenta tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Lane 6: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35/37 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721634) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (HA721634) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721634) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MSI2 antibody (HA721634) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721634) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells labeling MSI2 with Rabbit anti-MSI2 antibody (HA721634) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody (HA721634) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
MSI2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA721634 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721634 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA721634 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA721634 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 12 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"