ATP1A3 Recombinant Rabbit Monoclonal Antibody [JE35-24]
Catalog# HA721596
ATP1A3 Recombinant Rabbit Monoclonal Antibody [JE35-24]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ATP1A3 Recombinant Rabbit Monoclonal Antibody [JE35-24]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ATP1A3 aa 1-50 / 1,013.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 112 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, mouse cerebellum tissue lysate.
Conjugation
unconjugated
Clone Number
JE35-24
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Sodium/potassium-transporting ATPase subunit alpha-3 is an enzyme that in humans is encoded by the ATP1A3 gene. The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+-ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.[6] ATP1A3 is expressed early in human development, likely underlying pathophysiology related to several ATP1A3 related diseases.
Background References
1. Vezyroglou A et al. The Phenotypic Continuum of ATP1A3-Related Disorders. Neurology. 2022 Oct
2. Salles PA et al. ATP1A3-Related Disorders: An Ever-Expanding Clinical Spectrum. Front Neurol. 2021 Apr
Subcellular Location
Cell membrane.
Synonyms
AHC2 antibody
Alpha(III) antibody
AT1A3_HUMAN antibody
Atp1a3 antibody
ATPase Na+/K+ transporting alpha 3 polypeptide antibody
DYT 12 antibody
DYT12 antibody
MGC13276 antibody
Na(+)/K(+) ATPase alpha(III) subunit antibody
Na(+)/K(+) ATPase alpha-3 subunit antibody
ExpandImages
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Western blot analysis of ATP1A3 on different lysates with Rabbit anti-ATP1A3 antibody (HA721596) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse brain tissue lysate (70℃ heat)
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Rat brain tissue lysate
Lane 5: Rat brain tissue lysate (no heat)
Lane 6: Mouse cerebellum tissue lysate
Lane 7: Mouse cerebellum tissue lysate (70℃ heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 100/150 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721596) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"