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MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]

Usd: 385

Specification

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Catalog# HA721595

MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MT-ND1 aa 23-67 / 318.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Mouse heart tissue lysate, rat heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse smooth muscle tissue lysate.

Conjugation

unconjugated

Clone Number

JE34-93

RRID

AB_3072708

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

MT-ND1 is a gene of the mitochondrial genome coding for the NADH-ubiquinone oxidoreductase chain 1 (ND1) protein. The ND1 protein is a subunit of NADH dehydrogenase, which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of the human MT-ND1 gene are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.

Background References

1. Xu Y et al. Quantitative detection of circulating MT-ND1 as a potential biomarker for colorectal cancer. Bosn J Basic Med Sci. 2021 Oct

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: P03886 Human

SwissProt: P03888 Mouse

SwissProt: P03889 Rat

Synonyms

Complex I, subunit ND1 antibody

Mitochondrially encoded NADH dehydrogenase 1 antibody

MT-ND1 antibody

MTND1 antibody

NAD1 antibody

NADH dehydrogenase subunit 1 (complex I) antibody

NADH dehydrogenase subunit 1 antibody

NADH-ubiquinone oxidoreductase chain 1 antibody

NADH-ubiquinone oxidoreductase, subunit ND1 antibody

NADH1 antibody

Expand

Images

  • Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (<a href="/products/HA721595" style="font-weight: bold;text-decoration: underline;">HA721595</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse heart tissue lysate<br />Lane 2: Rat heart tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721595" style="font-weight: bold;text-decoration: underline;">HA721595</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (HA721595) at 1/1,000 dilution.

    Lane 1: Mouse heart tissue lysate
    Lane 2: Rat heart tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 37 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721595) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (<a href="/products/HA721595" style="font-weight: bold;text-decoration: underline;">HA721595</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate<br />Lane 2: Rat skeletal muscle tissue lysate<br />Lane 3: Mouse smooth muscle tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721595" style="font-weight: bold;text-decoration: underline;">HA721595</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (HA721595) at 1/1,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate
    Lane 3: Mouse smooth muscle tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 37 kDa

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721595) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

MT-ND1 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Rat

Conjugate: unconjugated