MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]
Catalog# HA721595
MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MT-ND1 Recombinant Rabbit Monoclonal Antibody [JE34-93]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MT-ND1 aa 23-67 / 318.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
Mouse heart tissue lysate, rat heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse smooth muscle tissue lysate.
Conjugation
unconjugated
Clone Number
JE34-93
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
MT-ND1 is a gene of the mitochondrial genome coding for the NADH-ubiquinone oxidoreductase chain 1 (ND1) protein. The ND1 protein is a subunit of NADH dehydrogenase, which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of the human MT-ND1 gene are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.
Background References
1. Xu Y et al. Quantitative detection of circulating MT-ND1 as a potential biomarker for colorectal cancer. Bosn J Basic Med Sci. 2021 Oct
Subcellular Location
Mitochondrion inner membrane.
Synonyms
Complex I, subunit ND1 antibody
Mitochondrially encoded NADH dehydrogenase 1 antibody
MT-ND1 antibody
MTND1 antibody
NAD1 antibody
NADH dehydrogenase subunit 1 (complex I) antibody
NADH dehydrogenase subunit 1 antibody
NADH-ubiquinone oxidoreductase chain 1 antibody
NADH-ubiquinone oxidoreductase, subunit ND1 antibody
NADH1 antibody
ExpandImages
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Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (HA721595) at 1/1,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 37 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721595) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (HA721595) at 1/1,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lane 3: Mouse smooth muscle tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 37 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721595) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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