XLF Recombinant Rabbit Monoclonal Antibody [PSH01-07]
Catalog# HA721572
XLF Recombinant Rabbit Monoclonal Antibody [PSH01-07]
-
WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
XLF Recombinant Rabbit Monoclonal Antibody [PSH01-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human XLF aa 1-250 / 299.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
HepG2 cell lysate, 293T cell lysate, NCCIT cell lysate, HeLa cell lysate, Jurkat cell lysate, human breast tissue, human colon tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
PSH01-07
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that in humans is encoded by the NHEJ1 gene. XLF was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Patients with XLF mutations also have immunodeficiency due to a defect in V(D)J recombination, which uses NHEJ to generate diversity in the antibody repertoire of the immune system. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the end-bridging or ligation steps of NHEJ. The yeast (Saccharomyces cerevisiae) homolog of XLF is Nej1. In contrast to the profound immunodeficiency phenotype of XLF deletion in humans, deletion of XLF alone has a mild phenotype in mice. However, combining a deletion of XLF with deletion of the ATM kinase causes a synthetic defect in NHEJ, suggesting partial redundancy in the function of these two proteins in mice.
Background References
1. Tang J et al. Role of Paralogue of XRCC4 and XLF in DNA Damage Repair and Cancer Development. Front Immunol. 2022 Mar
2. Carney SM et al. XLF acts as a flexible connector during non-homologous end joining. Elife. 2020 Dec
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
Cernunnos antibody
FLJ12610 antibody
NHEJ 1 antibody
Nhej1 antibody
NHEJ1, S. cerevisiae, homolog of antibody
NHEJ1_HUMAN antibody
Non homologous end joining factor 1 antibody
Non-homologous end-joining factor 1 antibody
Nonhomologous end joining factor 1 antibody
OTTHUMP00000164168 antibody
ExpandImages
-
Western blot analysis of XLF on different lysates with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: NCCIT cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (30 µg/Lane)
Lane 5: Jurkat cell lysate (30 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 37 kDa
Exposure time: 2 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721572) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721572) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721572) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-XLF antibody (HA721572) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721572) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"