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Phospholipase A2 Recombinant Rabbit Monoclonal Antibody [JE41-02]

Usd: 385

Specification

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Catalog# HA721565

Phospholipase A2 Recombinant Rabbit Monoclonal Antibody [JE41-02]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospholipase A2 Recombinant Rabbit Monoclonal Antibody [JE41-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Phospholipase A2 aa 95-144 / 144.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 16 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HepG2 cell lysate, rat liver tissue lysate, rat spleen tissue lysate, human liver tissue lysate, mouse liver tissue lysate, mouse spleen tissue lysate, mouse spleen tissue.

Conjugation

unconjugated

Clone Number

JE41-02

RRID

AB_3072681

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes. Simultaneously, by compromising mitochondrial membrane integrity, promotes the release in circulation of potent damage-associated molecular pattern molecules that activate the innate immune response. Plays a stem cell regulator role in the intestinal crypt. Within intracellular compartment mediates Paneth cell differentiation and its stem cell supporting functions by inhibiting Wnt signaling pathway in intestinal stem cell (ICS). Secreted in the intestinal lumen upon inflammation, acts in an autocrine way and promotes prostaglandin E2 synthesis that stimulates Wnt signaling pathway in ICS cells and tissue regeneration (By similarity). May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines.

Background References

1. Peng Z et al. Phospholipase A2 superfamily in cancer. Cancer Lett. 2021 Jan

Subcellular Location

Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane, Secreted

UNIPROT

SwissProt: P14555 Human

SwissProt: P31482 Mosue

SwissProt: P14423 Rat

Synonyms

EC 3.1.1.4 antibody

GIIC sPLA2 antibody

Group IIA phospholipase A2 antibody

membrane associated antibody

Modifier of MIN 1 mouse homolog antibody

MOM1 antibody

Non-pancreatic secretory phospholipase A2 antibody

NPS PLA2 antibody

NPS-PLA2 antibody

PA2GA_HUMAN antibody

Expand

Images

  • Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (<a href="/products/HA721565" style="font-weight: bold;text-decoration: underline;">HA721565</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br />Lane 6: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721565" style="font-weight: bold;text-decoration: underline;">HA721565</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)
    Lane 6: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 14 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (<a href="/products/HA721565" style="font-weight: bold;text-decoration: underline;">HA721565</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human liver tissue lysate (40 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 3: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 4: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721565" style="font-weight: bold;text-decoration: underline;">HA721565</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospholipase A2 on different lysates with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution.

    Lane 1: Human liver tissue lysate (40 µg/Lane)
    Lane 2: Mouse liver tissue lysate (40 µg/Lane)
    Lane 3: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 4: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 14 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospholipase A2 antibody (<a href="/products/HA721565" style="font-weight: bold;text-decoration: underline;">HA721565</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721565" style="font-weight: bold;text-decoration: underline;">HA721565</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospholipase A2 antibody (HA721565) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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