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DGAT1 Recombinant Rabbit Monoclonal Antibody [JE34-88]

Usd: 385

Specification

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Catalog# HA721555

DGAT1 Recombinant Rabbit Monoclonal Antibody [JE34-88]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DGAT1 Recombinant Rabbit Monoclonal Antibody [JE34-88]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DGAT1 aa 1-150 / 488.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 55 kDa

Positive Control

Human liver tissue lysate, MCF7 cell lysate, human liver tissue.

Conjugation

unconjugated

Clone Number

JE34-88

RRID

AB_3072671

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Diacylglycerol O-acyltransferase 1 is an enzyme that in humans is encoded by the DGAT1 gene. This gene encodes a multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. This enzyme is essential for lactation in mice, and mutations in this gene affect the composition and volume of milk produced by both cattle and goats. Without this gene activity, infants who have a mutation in this gene are incapable of breaking down fat. This lack of capability to break down fat causes diarrhea and vomiting which eventually causes FTT (Failure to Thrive) and need of TPN (Total Parenteral Nutrition) if not given correct formula. Further this will cause protein losing enteropathy and very low albumin.

Background References

1. Cheng X et al. Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress. Cell Metab. 2020 Aug

2. Zhou J et al. An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. Mol Cell. 2022 Aug

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: O75907 Human

Synonyms

ACAT related gene product 1 antibody

ACAT-related gene product 1 antibody

Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody

Acyl-CoA retinol O-fatty-acyltransferase antibody

Acyl-CoA:diacylglycerol acyltransferase antibody

ARAT antibody

ARGP1 antibody

C75990 antibody

D15Ertd23e antibody

Dgat antibody

Expand

Images

  • Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (<a href="/products/HA721555" style="font-weight: bold;text-decoration: underline;">HA721555</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human liver tissue lysate<br />Lane 2: MCF7 cell lysate (RIPA lysis)<br />Lane 3: MCF7 cell lysate (hot lysis)<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721555" style="font-weight: bold;text-decoration: underline;">HA721555</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (HA721555) at 1/1,000 dilution.

    Lane 1: Human liver tissue lysate
    Lane 2: MCF7 cell lysate (RIPA lysis)
    Lane 3: MCF7 cell lysate (hot lysis)

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 48 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721555) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (<a href="/products/HA721555" style="font-weight: bold;text-decoration: underline;">HA721555</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si DGAT1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721555" style="font-weight: bold;text-decoration: underline;">HA721555</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (HA721555) at 1/1,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si DGAT1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 48 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721555) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody (<a href="/products/HA721555" style="font-weight: bold;text-decoration: underline;">HA721555</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721555" style="font-weight: bold;text-decoration: underline;">HA721555</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody (HA721555) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721555) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation