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MAGOH Recombinant Rabbit Monoclonal Antibody [JE33-53]

Usd: 385

Specification

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Catalog# HA721548

MAGOH Recombinant Rabbit Monoclonal Antibody [JE33-53]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MAGOH Recombinant Rabbit Monoclonal Antibody [JE33-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MAGOH aa 97-146 / 146.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 17 kDa

Positive Control

HCT 116 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

JE33-53

RRID

AB_3072664

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Protein mago nashi homolog is a protein that in humans is encoded by the MAGOH gene. Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. MAGOH has been shown to interact with RBM8A and NXF1. In Drosophila melanogaster, Mago Nashi and Tsunagi/Y14 (core components of the exon junction complex) form a complex with a novel zinc finger protein, Ranshi, that has a role in oocyte differentiation.

Background References

1. Mitra R et al. Multifaceted roles of MAGOH Proteins. Mol Biol Rep. 2023 Feb

2. Soederberg A et al. MAGOH and MAGOHB Knockdown in Melanoma Cells Decreases Nonsense-Mediated Decay Activity and Promotes Apoptosis via Upregulation of GADD45A. Cells. 2022 Nov

Subcellular Location

Nucleus, Nucleus speckle, Cytoplasm.

UNIPROT

SwissProt: P61326 Human

SwissProt: P61327 Mouse

SwissProt: Q27W02 Rat

Synonyms

Mago nashi homolog proliferation associated (Drosophila) antibody

Mago nashi protein homolog antibody

magoh antibody

MAGOHA antibody

MGN_HUMAN antibody

Protein mago nashi homolog antibody

Images

  • Western blot analysis of MAGOH on different lysates with Rabbit anti-MAGOH antibody (<a href="/products/HA721548" style="font-weight: bold;text-decoration: underline;">HA721548</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: HepG2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721548" style="font-weight: bold;text-decoration: underline;">HA721548</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MAGOH on different lysates with Rabbit anti-MAGOH antibody (HA721548) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: HepG2 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 15 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721548) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAGOH antibody (<a href="/products/HA721548" style="font-weight: bold;text-decoration: underline;">HA721548</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721548" style="font-weight: bold;text-decoration: underline;">HA721548</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAGOH antibody (HA721548) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAGOH antibody (<a href="/products/HA721548" style="font-weight: bold;text-decoration: underline;">HA721548</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721548" style="font-weight: bold;text-decoration: underline;">HA721548</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAGOH antibody (HA721548) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721548) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"