CXCR6 Recombinant Rabbit Monoclonal Antibody [JE66-40]
Catalog# HA721542
CXCR6 Recombinant Rabbit Monoclonal Antibody [JE66-40]
-
WB
-
Human
-
unconjugated
Overview
Product Name
CXCR6 Recombinant Rabbit Monoclonal Antibody [JE66-40]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CXCR6 aa 1-100.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 39 kDa
Positive Control
JAR cell lysate, SW480 cell lysate, human placenta tissue lysate.
Conjugation
unconjugated
Clone Number
JE66-40
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
C-X-C chemokine receptor type 6 is a protein that in humans is encoded by the CXCR6 gene. CXCR6 has also recently been designated CD186 (cluster of differentiation 186). CXCR6 has been identified as an entry coreceptor used by HIV-1 and SIV to enter target cells, in conjunction with CD4. It is a minor coreceptor for HIV-1, nearly all strains of which use CCR5 and/or CXCR4. Most SIV strains can use CXCR6 and recent evidence suggests that in monkeys that serve as the natural hosts of SIV, CXCR6 may be a major coreceptor. CXCR6 was previously known as "Bonzo" and "STRL33" in the HIV/SIV field.
Background References
1. Di Pilato M et al. CXCR6 positions cytotoxic T cells to receive critical survival signals in the tumor microenvironment. Cell. 2021 Aug
2. Dudek M et al. Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH. Nature. 2021 Apr
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
bonzo antibody
C X C chemokine receptor type 6 antibody
C-X-C chemokine receptor type 6 antibody
CD 186 antibody
CD186 antibody
CD186 antigen antibody
CDw186 antibody
Chemokine (C X C motif) receptor 6 antibody
Chemokine C X C motif receptor 6 antibody
Chemokine CXC motif receptor 6 antibody
ExpandImages
-
Western blot analysis of CXCR6 on different lysates with Rabbit anti-CXCR6 antibody (HA721542) at 1/1,000 dilution.
Lane 1: JAR cell lysate (10 µg/Lane)
Lane 2: SW480 cell lysate (20 µg/Lane)
Lane 3: Human placenta tissue lysate (30 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721542) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"