TYRO3 Recombinant Rabbit Monoclonal Antibody [JE38-13]
Catalog# HA721539
TYRO3 Recombinant Rabbit Monoclonal Antibody [JE38-13]
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WB
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Human
-
Rat
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unconjugated
Overview
Product Name
TYRO3 Recombinant Rabbit Monoclonal Antibody [JE38-13]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TYRO3 aa 841-890 / 890.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 97 kDa
Positive Control
HeLa cell lysate, HCT 116 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, human brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE38-13
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses.
Background References
1. Jiang Z et al. TYRO3 induces anti-PD-1/PD-L1 therapy resistance by limiting innate immunity and tumoral ferroptosis. J Clin Invest. 2021 Apr
2. Engelmann J et al. Regulation of bone homeostasis by MERTK and TYRO3. Nat Commun. 2022 Dec
Subcellular Location
Cell membrane.
Synonyms
Brt antibody
BYK antibody
DTK antibody
Etk-2 antibody
protein tyrosine kinase 3 antibody
Rek antibody
RSE antibody
SKY antibody
Tif antibody
tyro3 antibody
ExpandImages
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Western blot analysis of TYRO3 on different lysates with Rabbit anti-TYRO3 antibody (HA721539) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HCT 116 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 100-150 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721539) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"