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c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]

Usd: 385

Specification

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Catalog# HA721514

c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human c-Myb aa 591-640 / 640.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 72 kDa

Positive Control

Jurkat cell lysate, HL-60 cell lysate.

Conjugation

unconjugated

Clone Number

JE40-07

RRID

AB_3072630

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Myb genes are part of a large gene family of transcription factors found in animals and plants. In humans, it includes Myb proto-oncogene like 1 and Myb-related protein B in addition to MYB proper. Members of the extended SANT/Myb family also include the SANT domain and other similar all-helical homeobox-like domains. The Myb gene family is named after the eponymous gene in Avian myeloblastosis virus. The viral Myb (v-Myb, P01104) recognizes the sequence 5'-YAACKG-3'. It causes myeloblastosis (myeloid leukemia) in chickens. Compared to the normal animal cellular Myb (c-myb), v-myb contains deletions in the C-terminal regulatory domain, leading to aberrant activation of other oncogenes.

Background References

1. Říhová K et al. Transcription factor c-Myb: novel prognostic factor in osteosarcoma. Clin Exp Metastasis. 2022 Apr

2. Bu C et al. c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway. Cell Death Discov. 2022 Feb

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P10242 Human

Synonyms

C myb antibody

c myb protein (140 AA) antibody

c-myb protein (140 AA) antibody

c-myb_CDS antibody

c-myb10A_CDS antibody

c-myb13A_CDS antibody

c-myb14A_CDS antibody

c-myb8B_CDS antibody

Cmyb antibody

efg antibody

Expand

Images

  • Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (<a href="/products/HA721514" style="font-weight: bold;text-decoration: underline;">HA721514</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: HL-60 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 72 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 3 minutes 6 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721514" style="font-weight: bold;text-decoration: underline;">HA721514</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA721514) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: HL-60 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

    Exposure time: 3 minutes 6 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721514) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"