TRPM7 Recombinant Rabbit Monoclonal Antibody [JE38-36]
Catalog# HA721501
TRPM7 Recombinant Rabbit Monoclonal Antibody [JE38-36]
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WB
-
FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TRPM7 Recombinant Rabbit Monoclonal Antibody [JE38-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TRPM7 aa 1,816-1,865 / 1,865.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 213 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, HCT 116 cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
JE38-36
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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FC
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1:500-1:1,000
Target
Function
Transient receptor potential cation channel, subfamily M, member 7, also known as TRPM7, is a human gene encoding a protein of the same name. TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants. TRPM7 has been shown to interact with PLCB1[6] and PLCB2.
Background References
1. Zhou R et al. TRPM7 channel inhibition attenuates rheumatoid arthritis articular chondrocyte ferroptosis by suppression of the PKCα-NOX4 axis. Redox Biol. 2022 Jul
2. Rios FJ et al. Chanzyme TRPM7 protects against cardiovascular inflammation and fibrosis. Cardiovasc Res. 2020 Mar
Subcellular Location
Membrane.
UNIPROT
Synonyms
ALSPDC antibody
CHAK antibody
CHAK1 antibody
Channel kinase 1 antibody
Channel-kinase 1 antibody
Long transient receptor potential channel 7 antibody
LTrpC-7 antibody
LTrpC7 antibody
Transient receptor potential cation channel subfamily M member 7 antibody
TRP PLIK antibody
ExpandImages
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Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (HA721501) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HCT 116 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 213 kDa
Observed band size: 250 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of HeLa cells labeling TRPM7.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721501, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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