SNF2H Recombinant Rabbit Monoclonal Antibody [JE40-44]
Catalog# HA721500
SNF2H Recombinant Rabbit Monoclonal Antibody [JE40-44]
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WB
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Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
SNF2H Recombinant Rabbit Monoclonal Antibody [JE40-44]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SNF2H aa 1,003-1,052 / 1,052.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 122 kDa
Positive Control
HeLa cell lysate, U-2 OS cell lysate, Jurkat cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE40-44
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 is a protein that in humans is encoded by the SMARCA5 gene. The protein encoded by this gene is a member of the ISWI family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. SMARCA5 has been shown to interact with RAD21, Histone deacetylase 2, POLE3, SATB1 and BAZ1A.
Background References
1. Bomber ML et al. Human SMARCA5 is continuously required to maintain nucleosome spacing. Mol Cell. 2023 Feb
2. Ding Y et al. Smarca5-mediated epigenetic programming facilitates fetal HSPC development in vertebrates. Blood. 2021 Jan
Subcellular Location
Nucleus, Chromosome.
Synonyms
4933427E24Rik antibody
D030040M08Rik antibody
D330027N15Rik antibody
EC 3.6.1.- antibody
hISWI antibody
hSNF2H antibody
ISWI antibody
MommeD4 antibody
SMARCA5 antibody
SMCA5_HUMAN antibody
ExpandImages
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Western blot analysis of SNF2H on different lysates with Rabbit anti-SNF2H antibody (HA721500) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 122 kDa
Observed band size: 122 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721500) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"