Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
Catalog# HA721492
Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
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WB
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Human
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Mouse
-
Rat
-
unconjugated
Overview
Product Name
Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Rad50 aa 1,162-1,312 / 1,312.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 154 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, U-87 MG cell lysate, U-937 cell lysate, Caco-2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
JE60-04
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
DNA repair protein RAD50, also known as RAD50, is a protein that in humans is encoded by the RAD50 gene. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1 (also known as Xrs2 in yeast). This MRN complex (MRX complex in yeast) binds to broken DNA ends and displays numerous enzymatic activities that are required for double-strand break repair by nonhomologous end-joining or homologous recombination. Gene knockout studies of the mouse homolog of Rad50 suggest it is essential for cell growth and viability. Two alternatively spliced transcript variants of Rad50, which encode distinct proteins, have been reported.
Background References
1. Kissling VM et al. Mre11-Rad50 oligomerization promotes DNA double-strand break repair. Nat Commun. 2022 May
2. Beikzadeh M et al. The dynamic nature of the Mre11-Rad50 DNA break repair complex. Prog Biophys Mol Biol. 2021 Aug
Subcellular Location
Nucleus, Chromosome, telomere.
Synonyms
DNA repair protein RAD50 antibody
hRAD50 antibody
hsRAD50 antibody
Mrell antibody
NBSLD antibody
Rad 50 antibody
RAD50 2 antibody
rad50 antibody
RAD50 homolog (S cerevisiae) antibody
RAD50 homolog antibody
ExpandImages
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Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (HA721492) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: U-937 cell lysate (20 µg/Lane)
Lane 5: Caco-2 cell lysate (20 µg/Lane)
Lane 6: Mouse testis tissue lysate (40 µg/Lane)
Lane 7: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 154 kDa
Observed band size: 154 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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