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Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]

Usd: 385

Specification

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Catalog# HA721486

Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Pumilio 2 aa 1-100.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 114 kDa

Positive Control

A549 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, U-2 OS.

Conjugation

unconjugated

Clone Number

JE37-76

RRID

AB_3072602

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants.

Background References

1. Elguindy MM et al. NORAD-induced Pumilio phase separation is required for genome stability. Nature. 2021 Jul

2. Silva ILZ et al. Modulation and function of Pumilio proteins in cancer. Semin Cancer Biol. 2022 Nov

Subcellular Location

Cytoplasm, Cytoplasmic granule, perinuclear region.

UNIPROT

SwissProt: Q8TB72 Human

Synonyms

FLJ36528 antibody

KIAA0235 antibody

MGC138251 antibody

MGC138253 antibody

PUM 2 antibody

PUM2 antibody

PUM2_HUMAN antibody

PUMH 2 antibody

PUMH2 antibody

Pumilio (Drosphila) homolog 2 antibody

Expand

Images

  • Western blot analysis of Pumilio 2 on different lysates with Rabbit anti-Pumilio 2 antibody (<a href="/products/HA721486" style="font-weight: bold;text-decoration: underline;">HA721486</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: U-2 OS cell lysate<br />Lane 3: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 114 kDa<br />Observed band size: 140 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721486" style="font-weight: bold;text-decoration: underline;">HA721486</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Pumilio 2 on different lysates with Rabbit anti-Pumilio 2 antibody (HA721486) at 1/1,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: U-2 OS cell lysate
    Lane 3: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 114 kDa
    Observed band size: 140 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721486) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of U-2 OS cells labeling Pumilio 2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721486" style="font-weight: bold;text-decoration: underline;">HA721486</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of U-2 OS cells labeling Pumilio 2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721486, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"