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Glutathione Peroxidase 2 Recombinant Rabbit Monoclonal Antibody [JE40-73]

Usd: 385

Specification

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Catalog# HA721485

Glutathione Peroxidase 2 Recombinant Rabbit Monoclonal Antibody [JE40-73]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Glutathione Peroxidase 2 Recombinant Rabbit Monoclonal Antibody [JE40-73]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C terminal Human Glutathione Peroxidase 2/GPX2.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 22 kDa

Positive Control

HT-29 cell lysate, HepG2 cell lysate, SW620 cell lysate, LoVo cell lysate, AGS cell lysate.

Conjugation

unconjugated

Clone Number

JE40-73

RRID

AB_3072601

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Glutathione peroxidase 2 is an enzyme that in humans is encoded by the GPX2 gene. This gene is a member of the glutathione peroxidase family encoding a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. The antioxidant enzyme glutathione peroxidase 2 (Gpx2) is one out of eight known glutathione peroxidases (Gpx1-8) in humans. Mammalian Gpx1, GPx2 (this protein), Gpx3, and Gpx4 have been shown to be selenium-containing enzymes, whereas Gpx6 is a selenoprotein in humans with cysteine-containing homologues in rodents. In selenoproteins, the 21st amino acid selenocysteine is inserted in the nascent polypeptide chain during the process of translational recoding of the UGA stop codon.

Background References

1. Yang M et al. GPX2 predicts recurrence-free survival and triggers the Wnt/β-catenin/EMT pathway in prostate cancer. PeerJ. 2022 Oct

2. Pu L et al. GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development. Animals (Basel). 2022 Dec

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: P18283 Human

Synonyms

Gastrointestinal glutathione peroxidase antibody

GI GPx antibody

Glutathione peroxidase 2 (gastrointestinal) antibody

Glutathione peroxidase 2 antibody

Glutathione peroxidase gastrointestinal antibody

Glutathione peroxidase related protein 2 antibody

Glutathione peroxidase-gastrointestinal antibody

Glutathione peroxidase-related protein 2 antibody

GPRP antibody

GPRP-2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody (<a href="/products/HA721485" style="font-weight: bold;text-decoration: underline;">HA721485</a>) at 1/2,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: HeLa cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721485" style="font-weight: bold;text-decoration: underline;">HA721485</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody (HA721485) at 1/2,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HeLa cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721485) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody (<a href="/products/HA721485" style="font-weight: bold;text-decoration: underline;">HA721485</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW620 cell lysate<br />Lane 2: LoVo cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: AGS cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 1 minute 17 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721485" style="font-weight: bold;text-decoration: underline;">HA721485</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody (HA721485) at 1/1,000 dilution.

    Lane 1: SW620 cell lysate
    Lane 2: LoVo cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: AGS cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 1 minute 17 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721485) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"