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CRALBP Recombinant Rabbit Monoclonal Antibody [PSH0-74]

Usd: 385

Specification

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Catalog# HA721483

CRALBP Recombinant Rabbit Monoclonal Antibody [PSH0-74]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CRALBP Recombinant Rabbit Monoclonal Antibody [PSH0-74]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CRALBP aa 1-317 / 317.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, IHC-Fr

Target Molecular Weight

Predicted band size: 36.5 kDa

Positive Control

SK-MEL-28 cell lysate, mouse eyeball tissue lysate, rat eyeball tissue lysate, mouse eyeball tissue, mouse retina tissue.

Conjugation

unconjugated

Clone Number

PSH0-74

RRID

AB_3072599

Reactivity Data

WB IHC-P IF-Tissue IHC-Fr
Human
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:500-1:1,000

  • IF-Tissue

  • 1:200

  • IHC-Fr

  • 1:500

Target

Function

Retinaldehyde-binding protein 1 (RLBP1) also known as cellular retinaldehyde-binding protein (CRALBP) is a 36-kD water-soluble protein that in humans is encoded by the RLBP1 gene. The cellular retinaldehyde-binding protein transports 11-cis-retinal (also known as 11-cis-retinaldehyde) as its physiological ligands. It plays a critical role as an 11-cis-retinal acceptor which facilitates the enzymatic isomerization of all 11-trans-retinal to 11-cis-retinal, in the isomerization of the rod and cones of the visual cycle. CRALBP is not just found in retina and retinal pigment epithelial cells, but also expressed in other cell types. It is majorly found in the iris, cornea, ciliary epithelium, Muller cells, the pineal gland and oligodendrocytes of the optic nerve and brain. This protein is also found in other tissues than the aforementioned ones, however its function in cells not related to the eyes are not yet known.

Background References

1. Chen DD et al. The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation. FASEB J. 2023 Mar

2. Lima de Carvalho JR Jr et al. Effects of deficiency in the RLBP1-encoded visual cycle protein CRALBP on visual dysfunction in humans and mice. J Biol Chem. 2020 May

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P12271 Human

SwissProt: Q9Z275 Mouse

Entrez Gene: 293049 Rat

Synonyms

Cellular retinaldehyde binding protein 1 antibody

Cellular retinaldehyde binding protein antibody

Cellular retinaldehyde-binding protein antibody

MGC3663 antibody

Retinaldehyde binding protein 1 antibody

Retinaldehyde-binding protein 1 antibody

RLBP 1 antibody

RLBP1 antibody

RLBP1_HUMAN antibody

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Retina<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Retina

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (<a href="/products/HA721483" style="font-weight: bold;text-decoration: underline;">HA721483</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-MEL-28 cell lysate<br />Lane 2: HepG2 cell lysate (negative cell)<br />Lane 3: Mouse eyeball tissue lysate<br />Lane 4: Rat eyeball tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 36.5 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721483" style="font-weight: bold;text-decoration: underline;">HA721483</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (HA721483) at 1/1,000 dilution.

    Lane 1: SK-MEL-28 cell lysate
    Lane 2: HepG2 cell lysate (negative cell)
    Lane 3: Mouse eyeball tissue lysate
    Lane 4: Rat eyeball tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 36.5 kDa
    Observed band size: 30 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721483) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-CRALBP antibody (<a href="/products/HA721483" style="font-weight: bold;text-decoration: underline;">HA721483</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721483" style="font-weight: bold;text-decoration: underline;">HA721483</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-CRALBP antibody (HA721483) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Retina<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-tissue

    Species: Mouse

    Site: Retina

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"