Ms4a10 Recombinant Rabbit Monoclonal Antibody [PSH0-71]
Catalog# HA721472
Ms4a10 Recombinant Rabbit Monoclonal Antibody [PSH0-71]
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WB
-
IHC-P
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IF-Tissue
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Mouse
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Rat
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unconjugated
Overview
Product Name
Ms4a10 Recombinant Rabbit Monoclonal Antibody [PSH0-71]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within mouse Ms4a10 aa 218-267 / 267.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
Mouse small intestine tissue, rat small intestine tissue.
Conjugation
unconjugated
Clone Number
PSH0-71
RRID
Reactivity Data
| WB | IHC-P | IF-Tissue | |
|---|---|---|---|
| Mouse |
|
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:100-1:500
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IF-Tissue
-
1:200
Target
Function
Most MS4A genes, including MS4A10, encode proteins with at least 4 potential transmembrane domains and N- and C-terminal cytoplasmic domains encoded by distinct exons. May be involved in signal transduction as a component of a multimeric receptor complex.
Background References
暂无
Subcellular Location
Membrane.
Synonyms
Membrane-spanning 4-domains subfamily A member 10
Ms4a10
Images
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☑ Cell treatment (CT)
Western blot analysis of Ms4a10 on different lysates with Rabbit anti-Ms4a10 antibody (HA721472) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa overexpress mouse Ms4a10 whole cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29/25 kDa
Exposure time: 4 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Ms4a10 antibody (HA721472) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721472) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Ms4a10 antibody (HA721472) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721472) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (HA721472) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721472, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (HA721472) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721472, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"