SMC2 Recombinant Rabbit Monoclonal Antibody [PSH0-62]
Catalog# HA721450
SMC2 Recombinant Rabbit Monoclonal Antibody [PSH0-62]
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WB
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IHC-P
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IF-Cell
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Human
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Green monkey
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unconjugated
Overview
Product Name
SMC2 Recombinant Rabbit Monoclonal Antibody [PSH0-62]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SMC2 aa 451-750 / 1,197.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 136 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, HepG2 cell lysate, A431 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, Raji cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human colon tissue, human colon lymph nodes tissue, HeLa, HT-29.
Conjugation
unconjugated
Clone Number
PSH0-62
RRID
Reactivity Data
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| Human |
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| Green Monkey |
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| Mouse |
|
|
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| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:100
Target
Function
Structural maintenance of chromosomes protein 2 (SMC-2), also known as chromosome-associated protein E (CAP-E), is a protein that in humans is encoded by the SMC2 gene. SMC2 is part of the SMC protein family and is a core subunit of condensin I and II, large protein complexes involved in chromosome condensation, overall organization. Several studies have demonstrated the necessity of SMC2 for cell division and proliferation. SMC2 works in the condensin complex as transcriptional regulation by compacting replicated DNA prior to mitotic division via supercoiling of the DNA. SMC2 also functions in resolving Sister chromatids prior to Anaphase.
Background References
1. Li X et al. Functions of SMC2 in the Development of Zebrafish Liver. Biomedicines. 2021 Sep
2. Montero S et al. Intracellular Delivery of Anti-SMC2 Antibodies against Cancer Stem Cells. Pharmaceutics. 2020 Feb
Subcellular Location
Nucleus, Cytoplasm, Chromosome.
UNIPROT
Synonyms
CAP E antibody
CAPE antibody
Chromosome associated protein E antibody
Chromosome-associated protein E antibody
FLJ10093 antibody
hCAP E antibody
hCAP-E antibody
hCAPE antibody
PRO0324 antibody
SMC 2 antibody
ExpandImages
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Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: HT-29 cell lysate (30 µg/Lane)
Lane 4: HepG2 cell lysate (30 µg/Lane)
Lane 5: A431 cell lysate (30 µg/Lane)
Lane 6: SH-SY5Y cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: Raji cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (24 µg/Lane)
Predicted band size: 136 kDa
Observed band size: 136 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721450) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721450) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721450) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon lymph nodes tissue with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721450) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of HT-29 cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"