MAD1L1 Recombinant Rabbit Monoclonal Antibody [JE35-54]
Catalog# HA721446
MAD1L1 Recombinant Rabbit Monoclonal Antibody [JE35-54]
-
WB
-
Human
-
unconjugated
Overview
Product Name
MAD1L1 Recombinant Rabbit Monoclonal Antibody [JE35-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human MAD1L1 aa 31-180 / 718.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
HCT 116 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Jurkat cell lysate, MCF7 cell lysate, A431 cell lysate, A375 cell lysate.
Conjugation
unconjugated
Clone Number
JE35-54
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. MAD1L1 is also known as Human Accelerated Region 3. It may have played a key role in the evolution of humans from apes. MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.
Background References
1. Liu X et al. MAD1L1 and TSNARE gene polymorphisms are associated with schizophrenia susceptibility in the Han Chinese population. BMC Med Genomics. 2021 Sep
2. Bandala-Jacques A et al. Prognostic Significance of the MAD1L1 1673 G: A Polymorphism in Ovarian Adenocarcinomas. Rev Invest Clin. 2020 Dec
Subcellular Location
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus.
UNIPROT
Synonyms
hMAD1 antibody
HsMAD1 antibody
MAD1 antibody
MAD1 mitotic arrest deficient like 1 (yeast) antibody
MAD1-like protein 1 antibody
MAD1L1 antibody
MD1L1_HUMAN antibody
Mitotic arrest deficient 1 antibody
Mitotic arrest deficient 1-like protein 1 antibody
Mitotic checkpoint MAD1 protein homolog antibody
ExpandImages
-
Western blot analysis of MAD1L1 on different lysates with Rabbit anti-MAD1L1 antibody (HA721446) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"