ZEB Recombinant Rabbit Monoclonal Antibody [PSH0-61] (HA721438)
Catalog# HA721438
Rabbit Monoclonal to ZEB
-
WB
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
Lane 1: Jurkat cell lysate
Lane 2: SK-OV-3 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: U87-MG cell lysate
Lane 6: MG-63 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 200 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721438) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ZEB Recombinant Rabbit Monoclonal Antibody [PSH0-61] (HA721438)
Immunogen
Recombinant protein within human zeb1 aa 1-400 / 1,124.
Host
Rabbit
Positive Control
Jurkat cell lysate, SK-OV-3 cell lysate, HeLa cell lysate, HEK-293 cell lysate, U87-MG cell lysate, MG-63 cell lysate, human colon tissue, human kidney tissue, human ovary carcinoma tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse stomach tissue, mouse testis tissue, rat kidney tissue, rat liver tissue, rat lung tissue, rat small intestine tissue, rat testis tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH0-61
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
-
FC
-
1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 124 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ZEB
SYNONYMS
AREB 6 antibody;AREB6 antibody;BZP antibody;Delta crystallin enhancer binding factor 1 antibody;DELTA EF1 antibody;FECD6 antibody;MGC133261 antibody;Negative regulator of IL 2 antibody;Negative regulator of IL2 antibody;NIL 2 A antibody;NIL 2 A zinc finger protein antibody;NIL 2A antibody;NIL-2-A zinc finger protein antibody;NIL2A antibody;Posterior polymorphous corneal dystrophy 3 antibody;PPCD3 antibody;Represses interleukin 2 expression antibody;TCF 8 antibody;TCF-8 antibody;TCF8 antibody;Transcription factor 8 (represses interleukin 2 expression) antibody;Transcription factor 8 antibody;ZEB 1 antibody;ZEB antibody;ZEB1 antibody;ZEB1_HUMAN antibody;ZFHEP antibody;ZFHX 1A antibody;ZFHX1A antibody;Zinc finger E box binding homeobox 1 antibody;Zinc finger E-box-binding homeobox 1 antibody;Zinc finger homeodomain enhancer binding protein antibody;FLJ42816 antibody;HSPC082 antibody;KIAA0569 antibody;SIP 1 antibody;SIP1 antibody;Smad Interacting Protein 1 antibody;Smad-interacting protein 1 antibody;SMADIP 1 antibody;SMADIP1 antibody;ZEB 2 antibody;Zeb2 antibody;ZEB2_HUMAN antibody;Zfhx1b antibody;ZFHX1B protein antibody;Zfx1b antibody;Zinc finger E box binding protein 2 antibody;Zinc finger E-box-binding homeobox 2 antibody;Zinc finger homeobox 1b antibody;zinc finger homeobox protein 1 antibody;Zinc finger homeobox protein 1b antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Zinc finger E-box-binding homeobox 1 is a protein that in humans is encoded by the ZEB1 gene. ZEB1 (previously known as TCF8) encodes a zinc finger and homeodomain transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. ZEB1 and its mammalian paralog ZEB2 belongs to the Zeb family within the ZF (zinc finger) class of homeodomain transcription factors. ZEB1 protein has 7 zinc fingers and 1 homeodomain. The structure of the homeodomain is shown on the right. Mutations of the gene are linked to posterior polymorphous corneal dystrophy 3. ZEB1 downregulates E-cadherin and induces epithelial to mesenchymal transition in breast and other carcinomas A recent study suggested its contributing role in lung cancer invasiveness and metastasis development. Overexpression of ZEB1 has been identified as a potential risk factor for recurrence and poor prognosis in several types of cancers.
CITATIONS
-
MiR-454-3p promotes apoptosis and autophagy of AML cells by targeting ZEB2 and regulating AKT/mTOR pathway
-
WB
-
Human
-
Citation
-
