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Desmocollin 1 Recombinant Rabbit Monoclonal Antibody [JE66-57]

Usd: 385

Specification

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Catalog# HA721418

Desmocollin 1 Recombinant Rabbit Monoclonal Antibody [JE66-57]

Application

  • WB

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Desmocollin 1 Recombinant Rabbit Monoclonal Antibody [JE66-57]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Desmocollin 1 aa 795-894 / 894.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 100 kDa

Positive Control

C2C12 cell lysate, L6 cell lysate, RH-35 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse heart tissue lysate, rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

JE66-57

RRID

AB_3072535

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Desmocollin-1 is a protein that in humans is encoded by the DSC1 gene. The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Alternative splicing results in two transcript variants encoding distinct isoforms. DSC1 has been shown to interact with Desmoglein 2.

Background References

1. Qie X et al. Role of DSC1 in Drosophila melanogaster synaptic activities in response to haedoxan A. Insect Sci. 2023 Feb

2. Zhang Z et al. CircRAB11FIP1 promoted autophagy flux of ovarian cancer through DSC1 and miR-129. Cell Death Dis. 2021 Feb

Subcellular Location

Cell membrane, Cell junction, desmosome.

UNIPROT

SwissProt: P55849 Mouse

Entrez Gene: 291759 Rat

Synonyms

Cadherin family member 1 antibody

CDHF1 antibody

Desmocollin1 antibody

Desmosomal glycoprotein 2/3 antibody

DG2/DG3 antibody

DSC 1 antibody

DSC1 antibody

Images

  • Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (<a href="/products/HA721418" style="font-weight: bold;text-decoration: underline;">HA721418</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 cell lysate<br />Lane 2: L6 cell lysate<br />Lane 3: RH-35 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 100 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721418" style="font-weight: bold;text-decoration: underline;">HA721418</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (HA721418) at 1/1,000 dilution.

    Lane 1: C2C12 cell lysate
    Lane 2: L6 cell lysate
    Lane 3: RH-35 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (<a href="/products/HA721418" style="font-weight: bold;text-decoration: underline;">HA721418</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Rat liver tissue lysate<br />Lane 3: Mouse heart tissue lysate<br />Lane 4: Rat heart tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 100 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721418" style="font-weight: bold;text-decoration: underline;">HA721418</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (HA721418) at 1/1,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate
    Lane 3: Mouse heart tissue lysate
    Lane 4: Rat heart tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"