Liver Carboxylesterase 1 Recombinant Rabbit Monoclonal Antibody [JE33-04]
Catalog# HA721416
Liver Carboxylesterase 1 Recombinant Rabbit Monoclonal Antibody [JE33-04]
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Liver Carboxylesterase 1 Recombinant Rabbit Monoclonal Antibody [JE33-04]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Liver Carboxylesterase 1 aa 51-100 / 567.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 62.5 kDa
Positive Control
U-937 cell lysate, human liver tissue lysate, human lung tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, human liver tissue, U-937.
Conjugation
unconjugated
Clone Number
JE33-04
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:100
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FC
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1:500-1:1,000
Target
Function
Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. The protein is also historically known as serine esterase 1 (SES1), monocyte esterase and cholesterol ester hydrolase (CEH). Three transcript variants encoding three different isoforms have been found for this gene. The various protein products from isoform a, b and c range in size from 568, 567 and 566 amino acids long, respectively. CES1 is present in most tissues with higher levels in the liver and low levels in the gastrointestinal tract. Carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. It is also part of the alpha/beta fold hydrolase family. These enzymes are responsible for the hydrolysis of ester- and amide-bond-containing xenobiotics and drugs such as cocaine and heroin. They also hydrolyze long-chain fatty acid esters and thioesters. As part of phase II metabolism, the resulting carboxylates are then often conjugated by other enzymes to increase solubility and eventually excreted. This enzyme is known to hydrolyze aromatic and aliphatic esters and can manage cellular cholesterol esterification levels. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds. The protein contains an amino acid sequence at its N-terminus that sends it into the endoplasmic reticulum where a C-terminal sequence can bind to a KDEL receptor.
Background References
1. Zhou L et al. Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. Clin Transl Med. 2021 Jun
2. Hernandez MH et al. CES1 and SLC6A2 Genetic Variants As Predictors of Response To Methylphenidate in Autism Spectrum Disorders. Pharmgenomics Pers Med. 2022 Nov
Subcellular Location
Endoplasmic reticulum lumen, Cytoplasm, Lipid droplet.
Synonyms
ACAT antibody
Acyl coenzyme A cholesterol acyltransferase antibody
Acyl-coenzyme A:cholesterol acyltransferase antibody
Brain carboxylesterase hBr1 antibody
Carboxyesterase ES-3 antibody
Carboxylesterase antibody
Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody
Carboxylesterase 1 antibody
Carboxylesterase 1 deficiency, included antibody
Carboxylesterase 2, formerly antibody
ExpandImages
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Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: Human liver tissue lysate (10 µg/Lane)
Lane 3: Human lung tissue lysate (10 µg/Lane)
Predicted band size: 62.5 kDa
Observed band size: 55 kDa
Exposure time: 16 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62.5 kDa
Observed band size: 55 kDa
Exposure time: 4 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721416) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of U-937 cells labeling Liver Carboxylesterase 1 with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of U-937 cells labeling Liver Carboxylesterase 1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721416, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"