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HB9/HLXB9/MNX1 Recombinant Rabbit Monoclonal Antibody [JE32-08]

Usd: 385

Specification

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Catalog# HA721410

HB9/HLXB9/MNX1 Recombinant Rabbit Monoclonal Antibody [JE32-08]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HB9/HLXB9/MNX1 Recombinant Rabbit Monoclonal Antibody [JE32-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HB9/HLXB9/MNX1 aa 276-325 / 401.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 41 kDa

Positive Control

HeLa cell lysate, Raji cell lysate, MCF7 cell lysate, HepG2 cell lysate, Huh7 cell lysate, PANC-1 cell lysate, SW1990 cell lysate, mouse pancreas tissue lysate.

Conjugation

unconjugated

Clone Number

JE32-08

RRID

AB_3072527

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Motor neuron and pancreas homeobox 1 (MNX1), also known as Homeobox HB9 (HLXB9), is a human protein encoded by the MNX1 gene. Mutations in the MNX1 gene are associated with Currarino syndrome. Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer.

Background References

1. Zhu Q et al. MNX1-AS1 Promotes Phase Separation of IGF2BP1 to Drive c-Myc-Mediated Cell-Cycle Progression and Proliferation in Lung Cancer. Cancer Res. 2022 Dec

2. Li T et al. LncRNA MNX1-AS1: A novel oncogenic propellant in cancers. Biomed Pharmacother. 2022 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P50219 Human

SwissProt: Q9QZW9 Mouse

SwissProt: M0R6D8 Rat

Synonyms

HB 9 antibody

HB9 antibody

HLXB 9 antibody

HLXB9 antibody

Homeo box HB9 antibody

Homeobox HB9 antibody

Homeobox protein HB9 antibody

HOXHB9 antibody

MNX1 antibody

MNX1_HUMAN antibody

Expand

Images

  • Western blot analysis of HB9/HLXB9/MNX1 on different lysates with Rabbit anti-HB9/HLXB9/MNX1 antibody (<a href="/products/HA721410" style="font-weight: bold;text-decoration: underline;">HA721410</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (15 µg/Lane)<br />Lane 2: Raji cell lysate (15 µg/Lane)<br />Lane 3: MCF7 cell lysate (15 µg/Lane)<br />Lane 4: HepG2 cell lysate (15 µg/Lane)<br />Lane 5: Huh7 cell lysate (15 µg/Lane)<br />Lane 6: PANC-1 cell lysate (15 µg/Lane)<br />Lane 7: SW1990 cell lysate (15 µg/Lane)<br />Lane 8: Mouse pancreas tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 5 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721410" style="font-weight: bold;text-decoration: underline;">HA721410</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HB9/HLXB9/MNX1 on different lysates with Rabbit anti-HB9/HLXB9/MNX1 antibody (HA721410) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (15 µg/Lane)
    Lane 2: Raji cell lysate (15 µg/Lane)
    Lane 3: MCF7 cell lysate (15 µg/Lane)
    Lane 4: HepG2 cell lysate (15 µg/Lane)
    Lane 5: Huh7 cell lysate (15 µg/Lane)
    Lane 6: PANC-1 cell lysate (15 µg/Lane)
    Lane 7: SW1990 cell lysate (15 µg/Lane)
    Lane 8: Mouse pancreas tissue lysate (30 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 5 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721410) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"