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HOXC6 Recombinant Rabbit Monoclonal Antibody [JE66-64]

Usd: 385

Specification

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Catalog# HA721409

HOXC6 Recombinant Rabbit Monoclonal Antibody [JE66-64]

Application

  • WB

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HOXC6 Recombinant Rabbit Monoclonal Antibody [JE66-64]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human HOXC6 aa 1-100 / 235.

Species Reactivity

Human, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 27 kDa

Positive Control

Raji cell lysate, Daudi cell lysate, HUVEC cell lysate, Jurkat cell lysate, PMVEC cell lysate, rat spinal cord tissue lysate, rat bladder tissue lysate.

Conjugation

unconjugated

Clone Number

JE66-64

RRID

AB_3072526

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Homeobox protein Hox-C6 is a protein that in humans is encoded by the HOXC6 gene. Hox-C6 expression is highest in the fallopian tube and ovary. HoxC6 has been highly expressed in many types of cancers including prostate, breast, and esophageal squamous cell cancer. This gene belongs to the homeobox family, members of which encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene, HOXC6, is one of several HOXC genes located in a cluster on chromosome 12. Three genes, HOXC5, HOXC4 and HOXC6, share a 5' non-coding exon. Transcripts may include the shared exon spliced to the gene-specific exons, or they may include only the gene-specific exons. Alternatively spliced transcript variants encoding different isoforms have been identified for HOXC6. Transcript variant two includes the shared exon, and transcript variant one includes only gene-specific exons. HOXC6 plays a role in lymphoma. The HOXC6 isoform, HOXC6-2 is an active carcinogenic for gastric cancer. It stimulates gastric cancer cells proliferation by acting as an oncogene. Downregulation of this gene’s isoforms could potentially lead to less proliferation of certain cancerous cells. With the HOXC6-1 isoform, there were no statistically significant effects on migration, invasion, apoptosis, or proliferation when it was downregulated. According to a study in Cancer Cell International, suppression of the HOXC6 gene plays a role in blocking the TGF-β/SMAD cascade. This then leads to the weakening of epithelial to mesenchymal transition for the cervical carcinoma.

Background References

1. Huang H et al. HOXC6 impacts epithelial-mesenchymal transition and the immune microenvironment through gene transcription in gliomas. Cancer Cell Int. 2022 Apr

2. Eryi S et al. HOXC6 Regulates the Epithelial-Mesenchymal Transition through the TGF-β/Smad Signaling Pathway and Predicts a Poor Prognosis in Glioblastoma. J Oncol. 2022 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P09630 Human

Entrez Gene: 252885 Rat

Synonyms

CP25 antibody

HHO.C8 antibody

Homeo box 3C antibody

Homeo box C6 antibody

Homeo box C8 protein antibody

Homeobox C6 antibody

Homeobox protein CP25 antibody

Homeobox protein HHO.C8 antibody

Homeobox protein Hox-3C antibody

Homeobox protein Hox-C6 antibody

Expand

Images

  • Western blot analysis of HOXC6 on different lysates with Rabbit anti-HOXC6 antibody (<a href="/products/HA721409" style="font-weight: bold;text-decoration: underline;">HA721409</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: Daudi cell lysate<br />Lane 3: HUVEC cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: PMVEC cell lysate<br />Lane 6: Rat spinal cord tissue lysate<br />Lane 7: Rat bladder tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 1 minute 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721409" style="font-weight: bold;text-decoration: underline;">HA721409</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HOXC6 on different lysates with Rabbit anti-HOXC6 antibody (HA721409) at 1/1,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: HUVEC cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: PMVEC cell lysate
    Lane 6: Rat spinal cord tissue lysate
    Lane 7: Rat bladder tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

    Exposure time: 1 minute 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721409) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"