Skip to content

Chromogranin B Recombinant Rabbit Monoclonal Antibody [JE31-72]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721398

Chromogranin B Recombinant Rabbit Monoclonal Antibody [JE31-72]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Chromogranin B Recombinant Rabbit Monoclonal Antibody [JE31-72]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Chromogranin B aa 601-650 / 677.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 78 kDa

Positive Control

293T cell lysate, SH-SY5Y cell lysate, SW620 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, HeLa cell lysate.

Conjugation

unconjugated

Clone Number

JE31-72

RRID

AB_3072515

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Secretogranin-1, also known as Chromogranin B, is a protein that in humans is encoded by the CHGB gene. It is a member of the granin protein family. Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.

Background References

1. Lyubimova NV et al. Chromogranin B in blood serum of patients with neuroendocrine tumors. Klin Lab Diagn. 2022 Aug

2. Jiang QX et al. The mechanisms of chromogranin B-regulated Cl- homeostasis. Biochem Soc Trans. 2022 Dec

Subcellular Location

Secreted.

UNIPROT

SwissProt: P05060 Human

SwissProt: P16014 Mouse

SwissProt: O35314 Rat

Synonyms

CCB peptide antibody

Cg B antibody

CgB antibody

Chgb antibody

Chromogranin-B antibody

ChromograninB antibody

SCG1 antibody

SCG1_HUMAN antibody

Secretogranin 1 antibody

Secretogranin B antibody

Expand

Images

  • Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (<a href="/products/HA721398" style="font-weight: bold;text-decoration: underline;">HA721398</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: SH-SY5Y cell lysate<br />Lane 3: SW620 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721398" style="font-weight: bold;text-decoration: underline;">HA721398</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: SW620 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 120 kDa

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (<a href="/products/HA721398" style="font-weight: bold;text-decoration: underline;">HA721398</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: RAW264.7 cell lysate<br />Lane 3: PC-12 cell lysate<br />Lane 4: HeLa cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721398" style="font-weight: bold;text-decoration: underline;">HA721398</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: RAW264.7 cell lysate
    Lane 3: PC-12 cell lysate
    Lane 4: HeLa cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 120 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"