KLKB1 Recombinant Rabbit Monoclonal Antibody [JE31-29]
Catalog# HA721397
KLKB1 Recombinant Rabbit Monoclonal Antibody [JE31-29]
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WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
KLKB1 Recombinant Rabbit Monoclonal Antibody [JE31-29]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human KLKB1 aa 589-638 / 638.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 71 kDa
Positive Control
Human liver tissue lysates, human liver tissue.
Conjugation
unconjugated
Clone Number
JE31-29
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:200
Target
Function
Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulfide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. Kallikrein and prekallikrein have been shown to interact with High-molecular-weight kininogen.
Background References
1. Che YQ et al. Serum KLKB1 as a Potential Prognostic Biomarker for Hepatocellular Carcinoma Based on Data-Independent Acquisition and Parallel Reaction Monitoring. J Hepatocell Carcinoma. 2021 Oct
2. Abraham RM et al. Prekallikrein deficiency due to homozygous KLKB1(+) mutation c.444_445insT (p.Ser151PhefsTer34). Int J Lab Hematol. 2022 Jun
Subcellular Location
Secreted.
UNIPROT
Synonyms
Fletcher factor antibody
kallikrein B plasma antibody
Kallikrein B plasma (Fletcher factor) 1 antibody
Kallikrein B, plasma 1 antibody
Kallikrein B1 antibody
Kininogenin antibody
KLK3 antibody
KLKB1 antibody
KLKB1_HUMAN antibody
PKK antibody
ExpandImages
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Western blot analysis of KLKB1 on human liver tissue lysates with Rabbit anti-KLKB1 antibody (HA721397) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721397) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-KLKB1 antibody (HA721397) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721397) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"