Skip to content

Dopamine Receptor D3 Recombinant Rabbit Monoclonal Antibody [JE33-33]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721391

Dopamine Receptor D3 Recombinant Rabbit Monoclonal Antibody [JE33-33]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Dopamine Receptor D3 Recombinant Rabbit Monoclonal Antibody [JE33-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Dopamine Receptor D3 aa 1-50 / 400.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 44 kDa

Positive Control

HeLa cell lysate, SH-SY5Y cell lysate, mouse brain tissue lysate, rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE33-33

RRID

AB_3072508

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Dopamine receptor D3 is a protein that in humans is encoded by the DRD3 gene. This gene encodes the D3 subtype of the dopamine receptor. The D3 subtype inhibits adenylyl cyclase through inhibitory G-proteins. This receptor is expressed in phylogenetically older regions of the brain, suggesting that this receptor plays a role in cognitive and emotional functions.[citation needed] It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson's disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Alpha-synuclein (α-Syn) aggregation via Lewy bodies inclusion, a pathogenic signature exclusively present in PD patients, is decreased by D3 agonists while DA content is elevated by inhibiting DA reuptake and breakdown. The regulation of α-Syn aggregation and clearance enhances brain-derived neurotrophic factor (BDNF) secretion, which ultimately ameliorates neuroinflammation and oxidative stress while promoting neurogenesis and interacting with other DA receptors. D3 agonists like 7-OH-DPAT, pramipexole, and rotigotine, among others, display antidepressant effects in rodent models of depression. Apomorphine has the ability to help PD patients with their cognition awareness. In addition to having antidepressant properties such as regulating the depression-like behaviors and depression development, pramipexole has the capability to prevent and slow down cell apoptosis as well as to restore damaged neural networks and connections while rotigotine help PD patients to attenuates hyperpyrexia syndrome and schizophrenia.

Background References

1. Pribiag H et al. Ventral pallidum DRD3 potentiates a pallido-habenular circuit driving accumbal dopamine release and cocaine seeking. Neuron. 2021 Jul

2. Liu C et al. DRD3 Ser9Gly polymorphism and treatment response to antipsychotics in schizophrenia: A meta-analysis. Neurosci Lett. 2022 Aug

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P35462 Human

SwissProt: P30728 Mouse

SwissProt: P19020 Rat

Synonyms

3dopamine receptor antibody

D antibody

D(3) dopamine receptor antibody

Dopamine D3 receptor antibody

Dopamine receptor D3 antibody

DRD3 antibody

DRD3_HUMAN antibody

ETM1 antibody

FET1 antibody

Images

  • Western blot analysis of Dopamine Receptor D3 on different lysates with Rabbit anti-Dopamine Receptor D3 antibody (<a href="/products/HA721391" style="font-weight: bold;text-decoration: underline;">HA721391</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (10 µg/Lane)<br />Lane 2: SH-SY5Y cell lysate (10 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 44 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721391" style="font-weight: bold;text-decoration: underline;">HA721391</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Dopamine Receptor D3 on different lysates with Rabbit anti-Dopamine Receptor D3 antibody (HA721391) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (10 µg/Lane)
    Lane 2: SH-SY5Y cell lysate (10 µg/Lane)
    Lane 3: Mouse brain tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 44 kDa
    Observed band size: 50 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation