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TFIIE alpha Recombinant Rabbit Monoclonal Antibody [JE66-41]

Usd: 385

Specification

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Catalog# HA721376

TFIIE alpha Recombinant Rabbit Monoclonal Antibody [JE66-41]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TFIIE alpha Recombinant Rabbit Monoclonal Antibody [JE66-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TFIIE alpha aa 390-439 / 439.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 49.5 kDa

Positive Control

K-562 cell lysate, Jurkat cell lysate, Daudi cell lysate, HeLa cell lysate, A431 cell lysate, A549 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, MCF7 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human colon cancer tissue, human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

JE66-41

RRID

AB_3072493

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

General transcription factor IIE subunit 1 (GTF2E1), also known as transcription initiation factor IIE subunit alpha (TFIIE-alpha), is a protein that in humans is encoded by the GTF2E1 gene. Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Background References

1. Phan T et al. Nucleolar TFIIE plays a role in ribosomal biogenesis and performance. Nucleic Acids Res. 2021 Nov

2. Ohkuma Y et al. Analysis of the role of TFIIE in basal transcription and TFIIH-mediated carboxy-terminal domain phosphorylation through structure-function studies of TFIIE-alpha. Mol Cell Biol. 1995 Sep

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P29083 Human

SwissProt: Q9D0D5 Mouse

Entrez Gene: 303918 Rat

Synonyms

FE antibody

General transcription factor IIE 56 kDa subunit antibody

General transcription factor IIE subunit 1 antibody

General transcription factor IIE, polypeptide 1, alpha 56kDa antibody

Gtf2e1 antibody

MAL7P1.86 antibody

T2EA_HUMAN antibody

TF2E1 antibody

TFIIE A antibody

TFIIE alpha antibody

Expand

Images

  • Western blot analysis of TFIIE alpha on different lysates with Rabbit anti-TFIIE alpha antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate (15 µg/Lane)<br />Lane 2: Jurkat cell lysate (15 µg/Lane)<br />Lane 3: Daudi cell lysate (15 µg/Lane)<br />Lane 4: HeLa cell lysate (15 µg/Lane)<br />Lane 5: A431 cell lysate (15 µg/Lane)<br />Lane 6: A549 cell lysate (15 µg/Lane)<br />Lane 7: HepG2 cell lysate (15 µg/Lane)<br />Lane 8: HEK-293 cell lysate (15 µg/Lane)<br />Lane 9: NIH/3T3 cell lysate (15 µg/Lane)<br />Lane 10: MCF7 cell lysate (15 µg/Lane)<br />Lane 11: Mouse testis tissue lysate (30 µg/Lane)<br />Lane 12: Rat testis tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 49.5 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TFIIE alpha on different lysates with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate (15 µg/Lane)
    Lane 2: Jurkat cell lysate (15 µg/Lane)
    Lane 3: Daudi cell lysate (15 µg/Lane)
    Lane 4: HeLa cell lysate (15 µg/Lane)
    Lane 5: A431 cell lysate (15 µg/Lane)
    Lane 6: A549 cell lysate (15 µg/Lane)
    Lane 7: HepG2 cell lysate (15 µg/Lane)
    Lane 8: HEK-293 cell lysate (15 µg/Lane)
    Lane 9: NIH/3T3 cell lysate (15 µg/Lane)
    Lane 10: MCF7 cell lysate (15 µg/Lane)
    Lane 11: Mouse testis tissue lysate (30 µg/Lane)
    Lane 12: Rat testis tissue lysate (30 µg/Lane)

    Predicted band size: 49.5 kDa
    Observed band size: 60 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721376) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TFIIE alpha antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721376) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TFIIE alpha antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721376) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TFIIE alpha antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721376) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TFIIE alpha antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721376" style="font-weight: bold;text-decoration: underline;">HA721376</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721376) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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