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E2F4 Recombinant Rabbit Monoclonal Antibody [JE40-78]

Usd: 385

Specification

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Catalog# HA721374

E2F4 Recombinant Rabbit Monoclonal Antibody [JE40-78]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

E2F4 Recombinant Rabbit Monoclonal Antibody [JE40-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human E2F4 aa 271-320 / 413.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 44 kDa

Positive Control

K-562 cell lysate, A549 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, HepG2 cell lysate, Raji cell lysate, HeLa cell lysate, human liver tissue, K-562.

Conjugation

unconjugated

Clone Number

JE40-78

RRID

AB_3072491

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Background References

1. Xiao W et al. Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy. 2022 Nov

2. Jin X et al. E2f4 is required for intestinal and otolith development in zebrafish. J Cell Physiol. 2022 Jun

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q16254 Human

Synonyms

E2F 4 antibody

E2F transcription factor 4 antibody

E2F transcription factor 4 p107/p130 binding antibody

E2F-4 antibody

E2F4 antibody

E2F4_HUMAN antibody

p107/p130 binding protein antibody

Transcription factor E2F 4 antibody

Transcription factor E2F4 antibody

Images

  • Western blot analysis of E2F4 on different lysates with Rabbit anti-E2F4 antibody (<a href="/products/HA721374" style="font-weight: bold;text-decoration: underline;">HA721374</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: HepG2 cell lysate<br />Lane 6: Raji cell lysate<br />Lane 7: HeLa cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721374" style="font-weight: bold;text-decoration: underline;">HA721374</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of E2F4 on different lysates with Rabbit anti-E2F4 antibody (HA721374) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: Raji cell lysate
    Lane 7: HeLa cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 60 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721374) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F4 antibody (<a href="/products/HA721374" style="font-weight: bold;text-decoration: underline;">HA721374</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721374" style="font-weight: bold;text-decoration: underline;">HA721374</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F4 antibody (HA721374) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721374) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of K-562 cells labeling E2F4.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721374" style="font-weight: bold;text-decoration: underline;">HA721374</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of K-562 cells labeling E2F4.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721374, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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