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Arp2 Recombinant Rabbit Monoclonal Antibody [JE40-62]

Usd: 385

Specification

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Catalog# HA721348

Arp2 Recombinant Rabbit Monoclonal Antibody [JE40-62]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Rat

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Arp2 Recombinant Rabbit Monoclonal Antibody [JE40-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Arp2 aa 345-394 / 394.

Species Reactivity

Human, Rat, Mouse

Validated Applications

WB, IHC-P, IF-Cell

Target Molecular Weight

Predicted band size: 45 kDa

Positive Control

Jurkat cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, human heart tissue, SH-SY5Y, rat spleen tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate.

Conjugation

unconjugated

Clone Number

JE40-62

RRID

AB_3072465

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:250

Target

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Background References

1. Chen D et al. Upregulation of Actin-Related Protein 2 (ACTR2) Exacerbated the Malignancy of Diffuse Large B-Cell Lymphoma through Activating Wnt Signaling. Comput Math Methods Med. 2022 Dec

2. Wang SR et al. miR-195 regulates intestinal epithelial restitution after wounding by altering actin-related protein-2 translation. Am J Physiol Cell Physiol. 2022 Apr

Subcellular Location

Cytoplasm, cytoskeleton, Cell projection, Nucleus.

UNIPROT

SwissProt: P61160 Human

SwissProt: P61161 Mouse

SwissProt: Q5M7U6 Rat

Synonyms

Actin like protein 2 antibody

Actin related protein 2 antibody

Actin-like protein 2 antibody

Actin-related protein 2 antibody

ACTR2 antibody

ARP2 actin related protein 2 homolog (yeast) antibody

ARP2 antibody

ARP2/3 COMPLEX antibody

ARP2_HUMAN antibody

OTTHUMP00000159937 antibody

Expand

Images

  • Western blot analysis of Arp2 on different lysates with Rabbit anti-Arp2 antibody (<a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate<br />Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 45 kDa<br />Observed band size: 40 kDa

    Western blot analysis of Arp2 on different lysates with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution.

    Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
    Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721348, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 45 kDa
    Observed band size: 40 kDa

  • Western blot analysis of Arp2 on Jurkat cell lysate with Rabbit anti-Arp2 antibody (<a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>) at 1/5,000 dilution.<br /><br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 44.8 kDa<br />Observed band size: 45 kDa

    Western blot analysis of Arp2 on Jurkat cell lysate with Rabbit anti-Arp2 antibody (HA721348) at 1/5,000 dilution.


    Lysates/proteins at 15 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721348, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 44.8 kDa
    Observed band size: 45 kDa

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Arp2 antibody (<a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721348) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling Arp2 with Rabbit anti-Arp2 antibody (<a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Arp2 antibody (<a href="/products/HA721348" style="font-weight: bold;text-decoration: underline;">HA721348</a>) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SH-SY5Y cells labeling Arp2 with Rabbit anti-Arp2 antibody (HA721348) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Arp2 antibody (HA721348) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

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