Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PS00-82] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PS00-82] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within full length human IL-1 beta.
Species Reactivity
Human, Dog
Validated Applications
ELISA(Det)
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
Recombinant IL-1 beta protein
Conjugation
unconjugated
Clone Number
PS00-82
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA (Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-39] to IL-1 beta (Capture) (HA721278).
Target
Function
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as Cryopyrin-Associated Periodic Syndromes (CAPS), due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1B. Intestinal dysbiosis has been observed to induce osteomyelitis through a IL-1β dependent manner. The presence of IL-1β has been also found in patients with multiple sclerosis (a chronic autoimmune disease of the central nervous system). However, it is not known exactly which cells produce IL-1β. Treatment of multiple sclerosis with glatiramer acetate or natalizumab has also been shown to reduce the presence of IL-1β or its receptor.
Background References
1. Qiao J et al. Autologous platelet rich plasma injection can be effective in the management of osteoarthritis of the knee: impact on IL-1 beta, TNF-alpha, hs-CRP. J Orthop Surg Res. 2024 Oct
2. Vicens-Artés S et al. Effect of MP-AzeFlu in IL-1 beta-induced IL-6 and proinflammatory cytokines. Immunol Res. 2023 Jun
Subcellular Location
Cytoplasm, Lysosome, Secreted
UNIPROT
Synonyms
Catabolin antibody
H1 antibody
IFN beta inducing factor antibody
IL 1 antibody
IL 1 beta antibody
IL-1 beta antibody
IL1 antibody
IL1 BETA antibody
IL1B antibody
IL1B_HUMAN antibody
ExpandImages
-
The binding activity of IL-1 beta (HA721279) with recombinant IL-1 beta protein.
Immobilized recombinant IL-1 beta protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721279) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Standard curve of IL-1 beta matched pair antibodies:<br /><br />Sandwich ELISA analysis of IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA721278" style="font-weight: bold;text-decoration: underline;">HA721278</a> [PS01-39] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IL-1 beta protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-82] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721279_2.jpg?v=20260908003456)
Standard curve of IL-1 beta matched pair antibodies:
Sandwich ELISA analysis of IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721278 [PS01-39] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IL-1 beta protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-82] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH16-86] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: unconjugated
Biotin Conjugated Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH16-86] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: Biotin
Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH16-87] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Dog
Conjugate: unconjugated
IL-1 beta Mouse Monoclonal Antibody [A7F9]
Application: WB,ELISA,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IL-1 beta Recombinant Rabbit Monoclonal Antibody [JJ087-3]
Application: WB,IF-Cell,IHC-P
Reactivity: Human
Conjugate: unconjugated
Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH16-85] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: unconjugated
IL-1 beta Recombinant Mouse Monoclonal Antibody [A7F7-R] - BSA and Azide free
Application: WB,ELISA,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IL-1 beta Recombinant Mouse Monoclonal Antibody [A7F7-R]
Application: WB,ELISA,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PS01-39] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Dog
Conjugate: unconjugated
IL-1 beta Mouse Monoclonal Antibody [A7A10]
Application: WB,ELISA,IF-Cell
Reactivity: Human
Conjugate: unconjugated
