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RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]

Usd: 385

Specification

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Catalog# HA721273

RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human RbBP5 aa 431-480 / 538.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 59 kDa

Positive Control

SW480 cell lysate, A431 cell lysate, C2C12 cell lysate, 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, K562 cell lysate, N2A cell lysate, PC-12 cell lysate, RAW264.7 cell lysate.

Conjugation

unconjugated

Clone Number

PSH0-15

RRID

AB_3072390

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Retinoblastoma-binding protein 5 is a protein that in humans is encoded by the RBBP5 gene. The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.

Background References

1. Xie H et al. Long non‑coding RNA AC245100.4 promotes prostate cancer tumorigenesis via the microRNA‑145‑5p/RBBP5 axis. Oncol Rep. 2021 Feb

2. Ali A et al. Diverse roles of WDR5-RbBP5-ASH2L-DPY30 (WRAD) complex in the functions of the SET1 histone methyltransferase family. J Biosci. 2017 Mar

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q15291 Human

SwissProt: Q8BX09 Mouse

Entrez Gene: 304794 Rat

Synonyms

RBBP 5 antibody

RBBP-5 antibody

RBBP5 antibody

RBBP5_HUMAN antibody

RBQ 3 antibody

RBQ3 antibody

Retinoblastoma binding protein 5 antibody

Retinoblastoma binding protein RBQ3 antibody

Retinoblastoma binding protein5 antibody

Retinoblastoma-binding protein 5 antibody

Expand

Images

  • Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (<a href="/products/HA721273" style="font-weight: bold;text-decoration: underline;">HA721273</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: C2C12 cell lysate<br />Lane 4: 293 cell lysate<br />Lane 5: MCF-7 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: K562 cell lysate<br />Lane 8: N2A cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: RAW264.7 cell lysate<br /><br />Lysates/proteins at 25 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721273" style="font-weight: bold;text-decoration: underline;">HA721273</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate
    Lane 2: A431 cell lysate
    Lane 3: C2C12 cell lysate
    Lane 4: 293 cell lysate
    Lane 5: MCF-7 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: K562 cell lysate
    Lane 8: N2A cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: RAW264.7 cell lysate

    Lysates/proteins at 25 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721273) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (<a href="/products/HA721273" style="font-weight: bold;text-decoration: underline;">HA721273</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH-3T3-si NT cell lysate<br />Lane 2: NIH-3T3-si RbBP5 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 4 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br /><a href="/products/HA721273" style="font-weight: bold;text-decoration: underline;">HA721273</a> was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721273" style="font-weight: bold;text-decoration: underline;">HA721273</a>, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution.

    Lane 1: NIH-3T3-si NT cell lysate
    Lane 2: NIH-3T3-si RbBP5 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: 4 minutes;

    4-20% SDS-PAGE gel.

    HA721273 was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721273, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"