p40 Recombinant Rabbit Monoclonal Antibody [PDH0-06]
Catalog# HA721255
p40 Recombinant Rabbit Monoclonal Antibody [PDH0-06]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
p40 Recombinant Rabbit Monoclonal Antibody [PDH0-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human p40 aa 2-50 / 586 (Q9H3D4-2)
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 77 kDa
Positive Control
A431 cell lysates, A431, rat skin tissue, human bladder carcinoma tissue, human lung squamous cell carcinoma tissue, human prostate tissue, human breast tissue, mouse bladder tissue.
Conjugation
unconjugated
Clone Number
PDH0-06
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:1,000-1:2,000
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FC
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1:500-1:1,000
Target
Function
p63 protein (p63) is a nuclear protein, a transcription factor. The p40 is an isoform of p63, a transcription factor that regulates many cell activities, including cell proliferation, maintenance, and differentiation. It performs as a sensitive and specific tool for aiding in the identification of squamous cell carcinoma of the lung. In addition to its utility as a squamous differentiation marker, p40 has also been proven to be a valuable marker for highlighting myoepithelial and basal cell populations in prostate, breast, skin, and salivary gland. Strong p40 expression is frequently observed in esophageal cancerous squamous lesions. Immunohistochemical detection of p40 can also be helpful in identifying urothelial carcinoma. In cases of prostate carcinoma, p40 is almost always found to be negative for basal cell staining. Among carcinomas, p40 has approximately the same sensitivity as p63 but a higher specificity, as the TAp63 isoform is expressed more widespread in eg., adenocarcinomas. Moreover, p63 occur in lymphomas that are p40 negative. Placenta is recommended as positive tissue control for p40, where an at least weak to moderate, distinct nuclear staining reaction of cytotrophoblasts must be seen. The cytotrophoblasts should be visible even at low magnification (5x objective). Supportive to placenta, tonsil can be used as positive and negative tissue control. Virtually all squamous epithelial cells must show a moderate to strong, distinct nuclear staining reaction. No nuclear or cytoplasmic staining reaction should be seen in other cell types.
Background References
1. Brustmann, Hermann. "p40 as a Basal Cell Marker in the Diagnosis of Prostate Glandular Proliferations: A Comparative Immunohistochemical Study with 34betaE12." Pathology research international vol. 2015 (2015): 897927.
2. Geddert, Helene et al. "The role of p63 and deltaNp63 (p40) protein expression and gene amplification in esophageal carcinogenesis." Human Pathology. 34,9 (2003): 850-856.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
40 kDa Rab9 effector protein antibody
8430412M01Rik antibody
9530020d24rik antibody
AV073337 antibody
bA65N13.1 antibody
C87311 antibody
DKFZp686P1077 antibody
OTTHUMP00000022126 antibody
OTTMUSP00000012898 antibody
OTTMUSP00000012899 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-p40 antibody (HA721255) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721255) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-p40 antibody (HA721255) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721255) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-p40 antibody (HA721255) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721255) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-p40 antibody (HA721255) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721255) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-p40 antibody (HA721255) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721255) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue with Rabbit anti-p40 antibody (HA721255) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of A431 cells labeling p40.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721255, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Western blot analysis of p40 on A431 cell lysate with Rabbit anti-p40 antibody (HA721255) at 1/1000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 60 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721255, 1/1000 in primary antibody dilution buffer (5% NFDM/TBST), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 76.8 kDa
Observed band size: 75 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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