Human IL-10 Recombinant Rabbit Monoclonal Antibody [PS00-81] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-10 Recombinant Rabbit Monoclonal Antibody [PS00-81] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length human IL-10 protein.
Species Reactivity
Human, Dog, Cynomolgus monkey
Validated Applications
ELISA(Det)
Target Molecular Weight
Predicted band size: 20 kDa
Conjugation
unconjugated
Clone Number
PS00-81
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration.
Target
Function
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively. IL-10 effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10 predominantly inhibits lipopolysaccharide (LPS) and bacterial product mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ secretion from Toll-Like Receptor (TLR) triggered myeloid lineage cells.
Background References
1. Mosser DM, Zhang X (December 2008). "Interleukin-10: new perspectives on an old cytokine". Immunological Reviews. 226 (1): 205–18.
2. Lazear HM, Nice TJ, Diamond MS (July 2015). "Interferon-λ: Immune Functions at Barrier Surfaces and Beyond". Immunity. 43 (1): 15–28.
Subcellular Location
Secreted
UNIPROT
Synonyms
CSIF antibody
Cytokine synthesis inhibitory factor antibody
GVHDS antibody
IL 10 antibody
IL-10 antibody
IL10 antibody
IL10_HUMAN antibody
IL10A antibody
Interleukin 10 antibody
Interleukin-10 antibody
ExpandImages
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![Sandwich ELISA analysis of IL-10 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA721236" style="font-weight: bold;text-decoration: underline;">HA721236</a> [PS00-98] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-10 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody <a href="/products/HA721237" style="font-weight: bold;text-decoration: underline;">HA721237</a> [PS00-81] (Biotin, 1:2000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-10 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.](https://storage.huabio.cn/huabio/productImg/HA721237_1.png?v=20260908003458)
Sandwich ELISA analysis of IL-10 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721236 [PS00-98] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-10 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721237 [PS00-81] (Biotin, 1:2000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-10 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human IL-10 Recombinant Rabbit Monoclonal Antibody [PS00-98] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: unconjugated
Biotin Conjugated Human IL-10 Recombinant Rabbit Monoclonal Antibody [PS00-81] - Detector
Application: ELISA(Det)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: Biotin
IL-10 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse
Conjugate: unconjugated
