RALA Recombinant Rabbit Monoclonal Antibody [JE54-12]
Catalog# HA721215
RALA Recombinant Rabbit Monoclonal Antibody [JE54-12]
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WB
-
IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
RALA Recombinant Rabbit Monoclonal Antibody [JE54-12]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human RALA aa 107-206 / 206.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
LOVO cell lysate, HCT116 cell lysate, HT-29 cell lysate, SW480 cell lysate, 293 cell lysate, human colon tissue, human colon carcinoma tissue, human kidney tissue, HUVEC.
Conjugation
unconjugated
Clone Number
JE54-12
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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FC
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1:500-1:1,000
Target
Function
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody. During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1.
Background References
1. Thies KA et al. The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer. Breast Cancer Res. 2021 Jun
2. Hussain SS et al. RalA and PLD1 promote lipid droplet growth in response to nutrient withdrawal. Cell Rep. 2021 Jul
Subcellular Location
Cell membrane, Mitochondrion.
UNIPROT
Synonyms
MGC48949 antibody
Ral a antibody
Ral A protein antibody
RAL antibody
RALA antibody
RALA_HUMAN antibody
Ras family small GTP binding protein RALA antibody
RAS like protein A antibody
Ras related protein RalA antibody
Ras-related protein Ral-A antibody
ExpandImages
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Western blot analysis of RALA on different lysates with Rabbit anti-RALA antibody (HA721215) at 1/1,000 dilution.
Lane 1: LoVo cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: SW480 cell lysate
Lane 5: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721215, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 24 kDa
Observed band size: 24 kDa -
☑ Knockdown (KD)
Western blot analysis of RALA on different lysates with Rabbit anti-RALA antibody (HA721215) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RALA KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721215) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RALA antibody (HA721215) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721215) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RALA antibody (HA721215) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RALA antibody (HA721215) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721215) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HUVEC cells labeling RALA.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721215, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
RALA Mouse Monoclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Monkey
Conjugate: unconjugated
