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VPAC1 Recombinant Rabbit Monoclonal Antibody [PD00-96]

Usd: 385

Specification

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Catalog# HA721209

VPAC1 Recombinant Rabbit Monoclonal Antibody [PD00-96]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

VPAC1 Recombinant Rabbit Monoclonal Antibody [PD00-96]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human VIPR1 aa 400-457.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 52 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, HT-29 cell lysate, Caco-2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, PC-12 cell lysate, LNCaP cell lysate, human tonsil tissue, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, C6 cell lysate, L6 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, mouse small intestine tissue lysate, mouse colon tissue lysate, rat lung tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

PD00-96

RRID

AB_3072329

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:500-1:4,000

Target

Function

There are two known receptors for the vasoactive intestinal peptide (VIP) termed VPAC1 and VPAC2. These receptors bind both VIP and pituitary adenylate cyclase-activating polypeptide (PACAP) to some degree. Both receptors are members of the 7 transmembrane G protein-coupled receptor family. VPAC1 is distributed widely in the CNS, liver, lung, intestine and T-lymphocytes. VPAC2 is found in the CNS, pancreas, skeletal muscle, heart, kidney, adipose tissue, testis, and stomach. Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) receptors are activated by the endogenous peptides VIP, PACAP-38, PACAP-27, peptide histidine isoleucineamide (PHI), peptide histidine methionineamide (PHM) and peptide histidine valine (PHV). Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene.

Background References

1. Tripathi SK et al. Targeting VPAC1 Receptors for Imaging Glioblastoma. Mol Imaging Biol. 2020 Apr

2. Palus K et al. Alterations in the relative abundance of the vasoactive intestinal peptide receptors (VPAC1 and VPAC2) and functions in uterine contractility during inflammation. Anim Reprod Sci. 2021 Feb

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P32241 Human

SwissProt: P97751 Mouse

SwissProt: P30083 Rat

Synonyms

FLJ41949 antibody

HVR 1 antibody

HVR1 antibody

PACAP R 2 antibody

PACAP type II receptor antibody

PACAP-R-2 antibody

PACAP-R2 antibody

Pituitary adenylate cyclase activating polypeptide receptor type II antibody

Pituitary adenylate cyclase activating polypeptide type II receptor antibody

Pituitary adenylate cyclase-activating polypeptide type II receptor antibody

Expand

Images

  • Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: HT-29 cell lysate (20 µg/Lane)<br />Lane 5: Caco-2 cell lysate (20 µg/Lane)<br />Lane 6: MCF7 cell lysate (20 µg/Lane)<br />Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 8: PC-12 cell lysate (20 µg/Lane)<br />Lane 9: LNCaP cell lysate (20 µg/Lane)<br /><br />Predicted band size: 52 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: HT-29 cell lysate (20 µg/Lane)
    Lane 5: Caco-2 cell lysate (20 µg/Lane)
    Lane 6: MCF7 cell lysate (20 µg/Lane)
    Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 8: PC-12 cell lysate (20 µg/Lane)
    Lane 9: LNCaP cell lysate (20 µg/Lane)

    Predicted band size: 52 kDa
    Observed band size: 55 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 2: C2C12 cell lysate (20 µg/Lane)<br />Lane 3: COS-1 cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: L6 cell lysate (20 µg/Lane)<br />Lane 6: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 7: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 8: Mouse small intestine tissue lysate (40 µg/Lane)<br />Lane 9: Mouse colon tissue lysate (40 µg/Lane)<br />Lane 10: Rat lung tissue lysate (40 µg/Lane)<br />Lane 11: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 52 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 2: C2C12 cell lysate (20 µg/Lane)
    Lane 3: COS-1 cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: L6 cell lysate (20 µg/Lane)
    Lane 6: Mouse liver tissue lysate (40 µg/Lane)
    Lane 7: Mouse lung tissue lysate (40 µg/Lane)
    Lane 8: Mouse small intestine tissue lysate (40 µg/Lane)
    Lane 9: Mouse colon tissue lysate (40 µg/Lane)
    Lane 10: Rat lung tissue lysate (40 µg/Lane)
    Lane 11: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 52 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-VPAC1 antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721209" style="font-weight: bold;text-decoration: underline;">HA721209</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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