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Tcl1 Recombinant Rabbit Monoclonal Antibody [PD00-94]

Usd: 385

Specification

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Catalog# HA721207

Tcl1 Recombinant Rabbit Monoclonal Antibody [PD00-94]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Tcl1 Recombinant Rabbit Monoclonal Antibody [PD00-94]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Tcl1 aa 1-114.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell, FC, IF-Tissue

Molecular Weight

Predicted band size: 13 kDa

Positive Control

Daudi cell lysate, Ramos cell lysate, human tonsil tissue, human appendix tissue, PC-3, Daudi.

Conjugation

unconjugated

Clone Number

PD00-94

RRID

AB_3072327

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:10,000-1:30,000

  • IF-Cell

  • 1:50

  • FC

  • 1:500-1:1,000

  • IF-Tissue

  • 1:1,000

Target

Function

T-cell leukemia/lymphoma protein 1A (TCL1) is a member of the TCL1 family and enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. TCL1 promotes the nuclear translocation of AKT1 and enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. The expression of TCL1 is restricted to lymphoid cells. It is expressed early in lymphocyte differentiation. Strong expression of TCL1 is found in a subset of mantle zone B lymphocytes and is expressed to a lesser extent by follicle center cells. In B-cell neoplasia, TCL1 immunoreactivity is found in the majority of B-cell lymphomas including lymphoblastic lymphoma, chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, Burkitt lymphoma, diffuse large B-cell lymphoma (60%), and primary cutaneous B-cell lymphoma (55%). The expression of the TCL1 gene characterizes low-grade B-cell lymphomas.

Background References

1. Sun S et al. Current understandings on T-cell prolymphocytic leukemia and its association with TCL1 proto-oncogene. Biomed Pharmacother. 2020 Jun

2. Lewis R et al. CXCR4 hyperactivation cooperates with TCL1 in CLL development and aggressiveness. Leukemia. 2021 Oct

Subcellular Location

Cytoplasm, Nucleus, Microsome, Endoplasmic reticulum.

UNIPROT

SwissProt: P56279 Human

Synonyms

Anti TCL1A antibody

Lymphoma/leukemia, T-cell antibody

Oncogene TCL-1 antibody

Oncogene TCL1 antibody

P14 TCL1 antibody

P14 TCL1 protein antibody

Protein p14 TCL1 antibody

T cell leukemia 1 antibody

T cell lymphoma 1 antibody

T cell lymphoma 1A antibody

Expand

Images

  • Western blot analysis of Tcl1 on different lysates with Rabbit anti-Tcl1 antibody (<a href="/products/HA721207" style="font-weight: bold;text-decoration: underline;">HA721207</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate<br />Lane 2: Ramos cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 13 kDa<br />Observed band size: 13 kDa<br /><br />Exposure time: 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721207" style="font-weight: bold;text-decoration: underline;">HA721207</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Tcl1 on different lysates with Rabbit anti-Tcl1 antibody (HA721207) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate
    Lane 2: Ramos cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

    Exposure time: 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Tonsil<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA721207" style="font-weight: bold;text-decoration: underline;">HA721207</a>, 1/30,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Tonsil
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA721207, 1/30,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Immunocytochemistry analysis of PC-3 cells labeling Tcl1 with Rabbit anti-Tcl1 antibody (<a href="/products/HA721207" style="font-weight: bold;text-decoration: underline;">HA721207</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tcl1 antibody (<a href="/products/HA721207" style="font-weight: bold;text-decoration: underline;">HA721207</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of PC-3 cells labeling Tcl1 with Rabbit anti-Tcl1 antibody (HA721207) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tcl1 antibody (HA721207) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of Daudi cells labeling Tcl1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721207" style="font-weight: bold;text-decoration: underline;">HA721207</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Daudi cells labeling Tcl1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: appendix<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: appendix

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

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