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SPARC Recombinant Rabbit Monoclonal Antibody [PD00-57]

Usd: 385

Specification

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Catalog# HA721199

SPARC Recombinant Rabbit Monoclonal Antibody [PD00-57]

Application

  • IHC-P

  • IF-Tissue

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

SPARC Recombinant Rabbit Monoclonal Antibody [PD00-57]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human SPARC aa 18-303.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue, WB

Target Molecular Weight

Predicted band size: 35 kDa

Positive Control

Human malignant melanoma tissue, human glioma tissue, A375 cell lysate, SH-SY5Y cell lysate.

Conjugation

unconjugated

Clone Number

PD00-57

RRID

AB_3072319

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P IF-Tissue
human
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:1,000

  • WB

  • 1:5,000

Target

Function

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Background References

1. Camacho D et al. SPARC-p53: The double agents of cancer. Adv Cancer Res. 2020

2. Veith C et al. SPARC, a Novel Regulator of Vascular Cell Function in Pulmonary Hypertension. Circulation. 2022 Mar

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

UNIPROT

SwissProt: P09486 Human

Synonyms

AA517111 antibody

Basement membrane protein 40 antibody

Basement-membrane protein 40 antibody

BM 40 antibody

BM-40 antibody

BM40 antibody

Cysteine rich protein antibody

hm:zeh0062 antibody

MGC128090 antibody

OI17 antibody

Expand

Images

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: glioma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: glioma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

  • Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SPARC antibody (<a href="/products/HA721199" style="font-weight: bold;text-decoration: underline;">HA721199</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721199" style="font-weight: bold;text-decoration: underline;">HA721199</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SPARC antibody (HA721199) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721199) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-SPARC antibody (<a href="/products/HA721199" style="font-weight: bold;text-decoration: underline;">HA721199</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721199" style="font-weight: bold;text-decoration: underline;">HA721199</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-SPARC antibody (HA721199) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721199) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (<a href="/products/HA721199" style="font-weight: bold;text-decoration: underline;">HA721199</a>) at 1/5,000 dilution.<br /><br />Lane 1: A375 cell lysate<br />Lane 2: SH-SY5Y cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: Lane 1: 59 seconds; Lane 2: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721199" style="font-weight: bold;text-decoration: underline;">HA721199</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (HA721199) at 1/5,000 dilution.

    Lane 1: A375 cell lysate
    Lane 2: SH-SY5Y cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 40 kDa

    Exposure time: Lane 1: 59 seconds; Lane 2: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721199) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"