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Fbxw7 Recombinant Rabbit Monoclonal Antibody [PD00-51]

Usd: 385

Specification

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Catalog# HA721198

Fbxw7 Recombinant Rabbit Monoclonal Antibody [PD00-51]

Application

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Fbxw7 Recombinant Rabbit Monoclonal Antibody [PD00-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Fbxw7 aa 1-707.

Species Reactivity

Human

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 80 kDa

Positive Control

Human bladder carcinoma tissue, human colon cancer tissue, human endometrial adenocarcinoma tissue.

Conjugation

unconjugated

Clone Number

PD00-51

RRID

AB_3072318

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200

Target

Function

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Cui D et al. FBXW7 Confers Radiation Survival by Targeting p53 for Degradation. Cell Rep. 2020 Jan

2. Fan J et al. Clinical significance of FBXW7 loss of function in human cancers. Mol Cancer. 2022 Mar

Subcellular Location

Chromosome, Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q969H0 Human

Synonyms

AGO antibody

Archipelago antibody

Archipelago homolog antibody

Archipelago, Drosophila, homolog of antibody

CDC4 antibody

CDC4, S.cerevisiae, homolog of antibody

DKFZp686F23254 antibody

F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody

F box and WD 40 domain protein 7 antibody

F box and WD repeat domain containing 7 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-Fbxw7 antibody (<a href="/products/HA721198" style="font-weight: bold;text-decoration: underline;">HA721198</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721198" style="font-weight: bold;text-decoration: underline;">HA721198</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-Fbxw7 antibody (HA721198) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721198) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Fbxw7 antibody (<a href="/products/HA721198" style="font-weight: bold;text-decoration: underline;">HA721198</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721198" style="font-weight: bold;text-decoration: underline;">HA721198</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Fbxw7 antibody (HA721198) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721198) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue with Rabbit anti-Fbxw7 antibody (<a href="/products/HA721198" style="font-weight: bold;text-decoration: underline;">HA721198</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721198" style="font-weight: bold;text-decoration: underline;">HA721198</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue with Rabbit anti-Fbxw7 antibody (HA721198) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721198) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"