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MAGEA1 Recombinant Rabbit Monoclonal Antibody [PD00-50]

Usd: 385

Specification

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Catalog# HA721197

MAGEA1 Recombinant Rabbit Monoclonal Antibody [PD00-50]

Application

  • WB

  • IHC-P

  • IP

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

MAGEA1 Recombinant Rabbit Monoclonal Antibody [PD00-50]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C terminus Human MAGE-A1.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP, IF-Tissue

Molecular Weight

Predicted band size: 34 kDa

Positive Control

A431 cell lysates, human malignant melanoma tissue, human testis tissue.

Conjugation

unconjugated

Clone Number

PD00-50

RRID

AB_3072317

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,500

  • IP

  • 1-2μg/sample

  • IF-Tissue

  • 1:200

Target

Function

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Background References

1. Zhong G et al. MAGEA1 and hTERT Peptide Treatment Improves the Potency of The Dendritic Cell- Cytotoxic T Lymphocytes (DC-CTL) Immunotherapy in DAC Treated Acute Myeloid Leukemia. J Cancer. 2022 Jan

2. Zhao J et al. MAGEA1 inhibits the expression of BORIS via increased promoter methylation. J Cell Sci. 2019 Jan

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P43355 Human

Synonyms

Antigen MZ2 E antibody

Antigen MZ2-E antibody

Cancer/testis antigen 1.1 antibody

cancer/testis antigen family 1, member 1 antibody

CT1.1 antibody

MAGA1_HUMAN antibody

MAGE 1 antigen antibody

MAGE-1 antigen antibody

MAGE1 antibody

MAGE1A antibody

Expand

Images

  • Western blot analysis of MAGEA1 on A431 cell lysates with Rabbit anti-MAGEA1 antibody (<a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MAGEA1 on A431 cell lysates with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 45 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721197) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-MAGEA1 antibody (<a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721197) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MAGEA1 antibody (<a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721197) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAGEA1 was immunoprecipitated from 0.2 mg A431 cell lysate with <a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A431 cell lysate (input)<br />Lane 2: <a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a> IP in A431 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA721197" style="font-weight: bold;text-decoration: underline;">HA721197</a> in A431 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    MAGEA1 was immunoprecipitated from 0.2 mg A431 cell lysate with HA721197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721197 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A431 cell lysate (input)
    Lane 2: HA721197 IP in A431 cell lysate
    Lane 3: Rabbit IgG instead of HA721197 in A431 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 seconds; ECL: K1801

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: testis<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: testis

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"