FOXP1 Recombinant Rabbit Monoclonal Antibody [PD00-47]
Catalog# HA721195
FOXP1 Recombinant Rabbit Monoclonal Antibody [PD00-47]
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IHC-P
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Human
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unconjugated
Overview
Product Name
FOXP1 Recombinant Rabbit Monoclonal Antibody [PD00-47]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human FOXP1 aa 350-450.
Species Reactivity
Human
Validated Applications
IHC-P
Molecular Weight
Predicted band size: 75 kDa
Positive Control
Human colon tissue, human lymph nodes tissue, human breast tissue.
Conjugation
unconjugated
Clone Number
PD00-47
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000-1:5,000
Target
Function
Forkhead box P1 (FOXP1) is a transcription factor normally expressed in many cellular processes and plays an important role in development, specifically in human leukocyte populations. Expression of FOXP1 can be found in neoplastic and non-neoplastic tissues. Overexpression of FOXP1 has been observed in a subset of diffuse large B-cell lymphoma (DLBCL), and thus FOXP1 immunohistochemistry has been shown to be helpful in the subclassification of DLBCL. Diffuse large B-cell lymphoma (DLBCL) most likely represents different clinicopathologic entities, which are difficult to separate using standard technique. Diffuse large B-cell lymphoma (DLBCL) can be divided into the subtypes of germinal center B-cell–like (GCB), activated B-cell–like (ABC), and unclassified DLBCL. The GCB and ABC subtypes have different pathogenetic mechanisms that will impact the development of targeted therapies. Despite the robustness of gene expression profiling (GEP) in subclassifying DLBCL, GEP techniques are not applicable to the routine clinical practice due to the substantial time, technological expertise, and scarce resources required. Therefore, it is beneficial for the translational application of the GEP classification into protein expression by tumor cells to be developed through immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded tissues. Different approaches using immunophenotypic algorithms with small panels of antibody biomarkers have been developed to translate the robust information from molecular studies into a routine clinical platform, using antibodies against CD10, BCL6, MUM1/IRF4, GCET1, FoxP1, LMO2, and BCL2. Therefore, FoxP1 is useful in subclassification of DLBCL and a high cutoff (≥80%) for FoxP1 is needed to achieve high specificity for the ABC subtype.
Background References
1. Lozano R et al. FOXP1 syndrome: a review of the literature and practice parameters for medical assessment and monitoring. J Neurodev Disord. 2021 Apr
2. Hu Z et al. miR-29c-3p inhibits autophagy and cisplatin resistance in ovarian cancer by regulating FOXP1/ATG14 pathway. Cell Cycle. 2020 Jan
Subcellular Location
Nucleus.
UNIPROT
Synonyms
12CC4 antibody
FLJ23741 antibody
Fork head related protein like B antibody
Forkhead box P1 antibody
Forkhead box protein P1 antibody
FOX P1 antibody
FOXP 1 antibody
foxp1 antibody
FOXP1_HUMAN antibody
Glutamine rich factor 1 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-FOXP1 antibody (HA721195) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-FOXP1 antibody (HA721195) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721195) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-FOXP1 antibody (HA721195) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"