Skip to content

Contact

(857) 353-6600

orders@huabio.com

6X His-tag, AlpHcAbs Rabbit Alpaca Monoclonal Antibody (HA721172)

Price: Regular price $385.00

Unit price per 

Product size

Bulk Order Quote   |   Customization Request

Catalog# HA721172

Alpaca Monoclonal to 6X His-tag, AlpHcAbs Rabbit

Tested for

  • WB

  • IP

  • ELISA

Specifications

Product Type

Alpaca monoclonal primary

Product Name

6X His-tag, AlpHcAbs Rabbit Alpaca Monoclonal Antibody (HA721172)

Immunogen

6*his tag fusion protein.

Host

Alpaca

Positive Control

C-terminal His-tag fusion protein lysate, N-terminal His-tag fusion protein lysate, Mid His-tag fusion protein lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

APPLICATION DILUTION

  • WB

  • 1:10,000-1:50,000 (C-His)

  • WB

  • 1:500-1:10,000 (N-His)

  • WB

  • 1:10,000-1:50,000 (Mid-His)

  • IP

  • 1:100-1:500

  • ELISA

  • 1:10,000-1:50,000

PROPERTIES

Form

Liquid

Storage Condition

Store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS pH=7.5

Concentration

1ug/ul

Purification

Protein A affinity purified.

TARGET

PROTEIN NAME

6X His-tag, AlpHcAbs Rabbit

SYNONYMS

6 His epitope tag antibody;Hexa His tag antibody;HHHHHH epitope tag antibody;HHHHHH tag antibody;His tag antibody;Polyhistidine Tag antibody

FUNCTION

His-Tag Antibody is a high quality monoclonal His-Tag antibody (also designated 6X His-Tag antibody or His-Probe antibody) suitable for the detection of the His-Tag protein. His-Tag Antibody is available as both the non-conjugated anti-His-Tag antibody form, as well as multiple conjugated forms of anti-His-Tag antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. Anti-His tag, AlpHcAbs Rabbit antibody is monovalentrecom mbinant single domain antibody fused to rabbit 1gG Fc,and Anti-His tag AlpHcAbs Rabbit antibody detects the His tag selectively,no re eactivity with other proteins.

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

Search

Main menu

Your cart is currently empty.
Shop now