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TMEM16A Recombinant Rabbit Monoclonal Antibody [PO00-27]

Usd: 385

Specification

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Catalog# HA721165

TMEM16A Recombinant Rabbit Monoclonal Antibody [PO00-27]

Application

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TMEM16A Recombinant Rabbit Monoclonal Antibody [PO00-27]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TMEM16A aa 400-500.

Species Reactivity

Human

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 114 kDa

Positive Control

Human gastrointestinal stromal tumor tissue, human gallbladder tissue, human breast tissue.

Conjugation

unconjugated

Clone Number

PO00-27

RRID

AB_3072287

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:1,500

Target

Function

Anoctamin-1 (ANO1) also known as Transmembrane member 16A (TMEM16A) is a protein that, in humans, is encoded by the ANO1 gene. Anoctamin-1 is a voltage-gated calcium-activated anion channel, which acts as a chloride channel and a bicarbonate channel. additionally Anoctamin-1 is apical iodide channel. It is expressed in smooth muscle, epithelial cells, vomeronasal neurons, olfactory sustentacular cells, and is highly expressed in interstitial cells of Cajal (ICC) throughout the gastrointestinal tract. ANO1 is a transmembrane protein that functions as a calcium-activated chloride channel. Ca2+, Sr2+, and Ba2+ activate the channel.

Background References

1. Hao A. et. al. Emerging Modulators of TMEM16A and Their Therapeutic Potential. J Membr Biol. 2021 Aug

2. Liu Y. et. al. The Ca2+-activated chloride channel ANO1/TMEM16A: An emerging therapeutic target for epithelium-originated diseases? Acta Pharm Sin B. 2021 Jun

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: Q5XXA6 Human

Synonyms

ANO 1 antibody

ANO1 antibody

ANO1_HUMAN antibody

Anoctamin 1 antibody

Anoctamin 1 calcium activated chloride channel antibody

Anoctamin-1 antibody

Anoctamin1 antibody

Ca2+ activated Cl- channel antibody

Calcium Activated Chloride Channel antibody

Discovered on gastrointestinal stromal tumors protein 1 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue with Rabbit anti-TMEM16A antibody (<a href="/products/HA721165" style="font-weight: bold;text-decoration: underline;">HA721165</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721165" style="font-weight: bold;text-decoration: underline;">HA721165</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue with Rabbit anti-TMEM16A antibody (HA721165) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721165) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-TMEM16A antibody (<a href="/products/HA721165" style="font-weight: bold;text-decoration: underline;">HA721165</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721165" style="font-weight: bold;text-decoration: underline;">HA721165</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-TMEM16A antibody (HA721165) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMEM16A antibody (<a href="/products/HA721165" style="font-weight: bold;text-decoration: underline;">HA721165</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721165" style="font-weight: bold;text-decoration: underline;">HA721165</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMEM16A antibody (HA721165) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721165) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"