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CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]

Usd: 385

Specification

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Catalog# HA721160

CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]

Application

  • IHC-P

  • IF-Tissue

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N Terminal human CD13.

Species Reactivity

Human, Mouse

Validated Applications

IHC-P, IF-Tissue, WB

Target Molecular Weight

Predicted band size: 110 kDa

Positive Control

Human tonsil tissue, human spleen tissue, human kidney tissue, mouse kidney tissue, PANC-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PD00-39

RRID

AB_3072282

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:20,000

  • IF-Tissue

  • 1:1,000

  • WB

  • 1:2,000

Target

Function

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma.

Background References

1. Ghosh M. et. al. CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis. Sci Rep. 2021 May

2. Lu C. et. al. CD13/Aminopeptidase N Is a Potential Therapeutic Target for Inflammatory Disorders. J Immunol. 2020 Jan

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P15144 Human

SwissProt: P97449 Mouse

Synonyms

Alanyl (membrane) aminopeptidase antibody

Alanyl aminopeptidase antibody

Aminopeptidase M antibody

Aminopeptidase N antibody

AMPN_HUMAN antibody

ANPEP antibody

AP M antibody

AP N antibody

AP-M antibody

AP-N antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD13 antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD13 antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD13 antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD13 antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: kidney<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Mouse

    Site: kidney

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/2,000 dilution.<br /><br />Lane 1: PANC-1 cell lysate (5 µg/Lane)<br />Lane 2: THP-1 cell lysate (30 µg/Lane)<br />Lane 3: U-937 cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (negative) (20 µg/Lane)<br />Lane 5: HL-60 cell lysate (20 µg/Lane)<br />Lane 6: Mouse kidney tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 110 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: Lane 1: 9 seconds; Lane 2-5: 24 seconds; Lane 6: 1 minute 45 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution.

    Lane 1: PANC-1 cell lysate (5 µg/Lane)
    Lane 2: THP-1 cell lysate (30 µg/Lane)
    Lane 3: U-937 cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (negative) (20 µg/Lane)
    Lane 5: HL-60 cell lysate (20 µg/Lane)
    Lane 6: Mouse kidney tissue lysate (20 µg/Lane)

    Predicted band size: 110 kDa
    Observed band size: 150 kDa

    Exposure time: Lane 1: 9 seconds; Lane 2-5: 24 seconds; Lane 6: 1 minute 45 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-CD13 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 110 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721160" style="font-weight: bold;text-decoration: underline;">HA721160</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-CD13 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 110 kDa
    Observed band size: 150 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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